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Expression of c-fos and c-jun proteins and AP-1 binding activity during cell cycle progression of HL60 cells and phytohemagglutinin-stimulated lymphocytes

Tay, DLM; Hoffbrand, AV; Wickremasinghe, RG; (1996) Expression of c-fos and c-jun proteins and AP-1 binding activity during cell cycle progression of HL60 cells and phytohemagglutinin-stimulated lymphocytes. In: EXPERIMENTAL HEMATOLOGY. (pp. 277 - 284). CARDEN JENNINGS PUBL CO LTD

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Abstract

The protein products of the c-fos (p62(c-fos)) and c-jun (p39(c-jun)) genes are members of the AP-1 transcription factor family and are thought to play important roles in the regulation of gene expression during the cell cycle. Most studies on the expression of these proteins in relation to the cell cycle have been performed at the mRNA level, and therefore do not give direct information about the presence of the proteins during the cell cycle. We have used Western blotting to investigate the presence of these proteins during the cell cycles of two different cellular systems: a continuously growing myeloid leukemic cell line, HL60, and normal cells stimulated into cycle, phytohemagglutinin (PHA)-stimulated normal human peripheral blood lymphocytes (PBL). The binding activity of transcription factor AP-1, which consists of dimers of Fos and Jun family proteins, was also studied using a gel shift assay. We found nuclear p62(c-fos), p34(c-jun), and AP-1 binding activity throughout the cell cycle both in HL60 cells and in PHA-stimulated PBL, and we postulate that these proteins are required throughout the cell cycle and not transiently in the G(0) to G(1) transition as previous mRNA studies have indicated. We demonstrated an uncoupling of AP-1 binding activity from p62(c-fos), and p39(c-jun) AP-1 activity was expressed more strongly in the G(1-) and G(2)/M-phase enriched samples than in the S-phase enriched samples of HL60 cells, while levels of nuclear p62(c-fos) and p39(c-jun) were constant. Nuclei of unstimulated PBL from different donors expressed p62(c-fos) and p39(c-jun), but AP-1 was not detected in the majority of samples. Following PHA stimulation of PBL, the increase in AP-1 activity was delayed with respect to the augmentation of p39(c-jun) expression. We also observed that cytoplasmic p62(c-fos) and p39(c-jun) were present in HL60 cells and PHA-stimulated PBL. However, no cytoplasmic p62(c-fos) was detected in unstimulated PBL, although in some cases cytoplasmic p39(c-jun) was detected, suggesting that subcellular compartmentalization of these proteins may occur under certain circumstances.

Type: Proceedings paper
Title: Expression of c-fos and c-jun proteins and AP-1 binding activity during cell cycle progression of HL60 cells and phytohemagglutinin-stimulated lymphocytes
Event: 1995 Annual Meeting of the ISEH
Location: DUSSELDORF, GERMANY
Dates: 1995
Keywords: cell cycle, phytohemagglutinin, HL60 cells, lymphocytes, centrifugal elutriation, PROTO-ONCOGENE, MOUSE FIBROBLASTS, G0-TO-G1 TRANSITION, SIGNAL TRANSDUCTION, PHOSPHORYLATION, INDUCTION, INVITRO, GROWTH, TRANSCRIPTION, LOCALIZATION
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute > Research Department of Haematology
URI: http://discovery.ucl.ac.uk/id/eprint/121815
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