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Ionic currents expressed in a cell line derived from the organ of Corti of the Immortomouse

Jagger, DJ; Holley, MC; Ashmore, JF; (1999) Ionic currents expressed in a cell line derived from the organ of Corti of the Immortomouse. PFLUG ARCH EUR J PHY , 438 (1) 8 - 14.

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Abstract

We have investigated the maturation of adult hair cell electrophysiology in a population of precursor cells in a conditionally immortal cell line. The cell line, UB/OC-2, from the embryonic organ of Corti of the H-2Kb-tsA58 transgenic mouse. permits cells to grow proliferatively at 33 degrees C and to differentiate at 39 degrees C. Whole-cell patch-clamp recordings showed that proliferating cells had a different electrophysiology to differentiating cells. Differentiating cells had a conditionally expressed slowly activating inward current activated by hyperpolarization. The current was not blocked by extracellular application of 0.5 mM Ba2+, but was bloch-ed reversibly by 2 mM Cs+. The current was found to be carried by both K+ and Na+ ions (P-K/P-Na = 2.2) and activated by 10 mu M forskolin. These properties identify the slowly activating current as I-h. A proportion of proliferating and differentiating cells exhibited a voltage-gated Na+ current, I-Na. I-Na was abolished in Na-free external medium and was inhibited reversibly by tetrodotoxin (TTX) with K-i=64 nM. Together these results suggest that proliferating and differentiating hair cell precursors in the immortal cochlear cell line UB/OC-2 express currents which are also found in developing hair cells.

Type: Article
Title: Ionic currents expressed in a cell line derived from the organ of Corti of the Immortomouse
Keywords: cell lines, cochlea, development, hair cell, tsA58, whole cell currents, COCHLEAR HAIR-CELLS, ACTIVATED CATION CURRENT, SODIUM-CHANNELS, POSTNATAL-DEVELOPMENT, ACTION-POTENTIALS, NEURONS, CONDUCTANCE, MECHANISMS, MICE
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > The Ear Institute
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Neuro, Physiology and Pharmacology
URI: http://discovery.ucl.ac.uk/id/eprint/121726
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