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STUDIES INVIVO AND INVITRO OF SERUM AMYLOID-P-COMPONENT IN NORMALS AND IN A PATIENT WITH AA AMYLOIDOSIS

HAWKINS, PN; TENNENT, GA; WOO, P; PEPYS, MB; (1991) STUDIES INVIVO AND INVITRO OF SERUM AMYLOID-P-COMPONENT IN NORMALS AND IN A PATIENT WITH AA AMYLOIDOSIS. CLIN EXP IMMUNOL , 84 (2) 308 - 316.

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Abstract

Pure serum amyloid P component (SAP) was isolated from a normal donor pool, from individuals with the different genotypes of an MspI restriction fragment length polymorphism (RFLP) linked to the SAP gene, and from a patient with AA amyloidosis. The SAP preparations were all identical and all behaved as a single homogeneous species in polyacrylamide gel electrophoresis, isoelectric focussing, reverse-phase chromatography, binding in vitro to phosphoethanolamine-Sepharose (binding constant 2.4 x 10(7) l/mol) and AL amyloid fibrils (1.6 x 10(8) l/mol), and binding to amyloid deposits in vivo in mice with casein-induced amyloidosis. The in vivo metabolism of I-125-SAP from a single donor was normal and identical in three healthy individuals representing the three different MspI RFLP genotypes. There is thus no frequent polymorphism of SAP in normal subjects, and SAP altered with respect to the characteristics studied here is not a necessary condition for pathogenesis of systemic AA amyloidosis.

Type: Article
Title: STUDIES INVIVO AND INVITRO OF SERUM AMYLOID-P-COMPONENT IN NORMALS AND IN A PATIENT WITH AA AMYLOIDOSIS
Keywords: SERUM AMYLOID-P-COMPONENT, AMYLOIDOSIS, POLYMORPHISM, C-REACTIVE PROTEIN, BOVINE SERUM, DEPOSITS, SAP, IDENTIFICATION, INJECTIONS, BINDING, MOUSE
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Infection and Immunity
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine > Inflammation
URI: http://discovery.ucl.ac.uk/id/eprint/121599
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