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Receptor Arrays for the Selective and Efficient Capturing of Viral Particles

Pollheimer, PD; Kastner, M; Ebner, A; Blaas, D; Hinterdorfer, P; Gruber, HJ; Howorka, S; (2009) Receptor Arrays for the Selective and Efficient Capturing of Viral Particles. BIOCONJUGATE CHEM , 20 (3) 466 - 475. 10.1021/bc800357j.

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Abstract

We describe microarrays of receptors on gold/glass substrates for the selective capturing of viral particles at high density. Microscale gold squares were surface-modified with alkanethiol derivatives which enabled the immobilization of the HiS(6)-tagged virus-binding domain from the very-low density lipoprotein (VLDL) receptor. The free glass areas surrounding the gold squares were passivated with a dense film of poly(ethylene glycol) (PEG). As assessed by atomic force microscopy, human rhinovirus particles were captured onto the VLDL-receptor patches with a high surface coverage but were effectively repelled by the PEG layer, resulting in a 330 000-fold higher density of the particles on the gold as compared to the glass surfaces. The metal chelate-based coupling strategy was found to be superior to two alternative routes, which used the covalent coupling of viral particles or viral receptors to the substrate surface. The high-density receptor arrays were employed for sensing and characterizing viral particles with so far unprecedented selectivity.

Type: Article
Title: Receptor Arrays for the Selective and Efficient Capturing of Viral Particles
DOI: 10.1021/bc800357j
Keywords: SELF-ASSEMBLED MONOLAYERS, DENSITY POLY(ETHYLENE GLYCOL), FORCE MICROSCOPY, POLY(L-LYSINE)-G-POLY(ETHYLENE GLYCOL), OXIDE SURFACES, MOSAIC-VIRUS, COMMON COLD, PROTEIN, GOLD, IMMOBILIZATION
UCL classification: UCL > School of BEAMS > Faculty of Maths and Physical Sciences > Chemistry
URI: http://discovery.ucl.ac.uk/id/eprint/120836
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