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Interaction of androgen and 1,25-dihydroxyvitamin D3: effects on normal rat bone cells.

Gray, C; Colston, KW; Mackay, AG; Taylor, ML; Arnett, TR; (1992) Interaction of androgen and 1,25-dihydroxyvitamin D3: effects on normal rat bone cells. J Bone Miner Res , 7 (1) pp. 41-46. 10.1002/jbmr.5650070107.

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Abstract

We studied the actions of testosterone (T) and 5 alpha-dihydrotestosterone (DHT) in combination with 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3] on primary rat bone cells. The actions of androgens were generally anabolic, although response patterns varied considerably between cultures. For example, DHT caused striking dose- and time-dependent increases in [3H]thymidine incorporation into calvarial cells over the range 1-100 nM, with maximal stimulation of 2.5-fold after 9 days in culture. Testosterone (50 nM) also stimulated [3H]thymidine incorporation into long bone-derived cells. 1,25-(OH)2D3 generally blunted or abolished the proliferative action of androgens but was not itself always inhibitory; in some experiments, stimulation of [3H]thymidine incorporation occurred. Collagen production, as assessed by [3H]proline incorporation into pepsin-resistant protein secreted by calvarial cells, was also stimulated by DHT. In some cultures, androgen responses were absent, although striking inhibitory responses to 1,25-(OH)2D3 were observed. These results illustrate complex patterns of responses to androgens and 1,25-(OH)2D3 in cells derived from rat bone.

Type: Article
Title: Interaction of androgen and 1,25-dihydroxyvitamin D3: effects on normal rat bone cells.
Location: United States
DOI: 10.1002/jbmr.5650070107
Keywords: Alkaline Phosphatase, Animals, Bone and Bones, Calcitriol, Cell Division, Cells, Cultured, Collagen, Dihydrotestosterone, Dose-Response Relationship, Drug, Male, Rats, Rats, Inbred Strains, Testosterone
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Cell and Developmental Biology
URI: http://discovery.ucl.ac.uk/id/eprint/120453
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