Rashid, ST; Salacinski, HJ; Button, MJC; Fuller, B; Hamilton, G; Seifalian, AM; (2004) Cellular engineering of conduits for coronary and lower limb bypass surgery: Role of cell attachment peptides and pre-conditioning in optimising smooth muscle cells (SMC) adherence to compliant poly(carbonate-urea)urethane (MyoLink((TM))) scaffolds. EUR J VASC ENDOVASC , 27 (6) 608 - 616. 10.1016/j.ejvs.2004.01.020.
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Objective. We are developing a hybrid arterial bypass graft of compliant poly(carbonate-urea)urethane (MyoLink(TM)), endothelial and smooth muscle cells (SMCs). To enhance adhesion of SMCs we assessed various attachment factors and the effect of pre-conditioning on cell retention.Methods. MyoLink segments were coated with either RGD, superfibronectin, fibronectin, fibronectin-like engineered polymer protein (FEPP), FEPP plus or type 1 collagen overnight. (111)Indium-radiolabelled SMCs were placed onto MyoLink segments for 48 h before being aspirated, then lavaged off. All grafts, aspirates and lavages were counted in a gamma counter. SMC viability on the MyoLink segments was also assessed for viability using the Alamar blue redox assay. Separately, MyoLink grafts lined. with radiolabelled SMCs were divided into a pre-conditioned group, exposed to subarterial pulsatile flow whilst another group were held in static culture. After 1-week, grafts were exposed to arterial pulsatile flow whilst radioactivity was assessed using a gamma camera.Results. Only FEPP plus significantly enhanced SMC attachment: mean of 32 +/- 6% cell attachment compared to 21 +/- 5% for uncoated control. Cell viability was enhanced by all attachment factors except fibronectin. Pre-conditioning was shown to significantly enhance the retention of SMCs onto the MyoLink once exposed to pulsatile arterial flow:the final attachment was 57 +/- 7% for the static and 76 +/- 7% for the pre-conditioned group.Conclusions. FEPP plus enhances SMC attachment to MyoLink. We believe this is because of its repeating sequences of RGD and its positive charge. Pre-conditioning enhances the retention of SMCs to MyoLink once exposed to pulsatile arterial flow.
|Title:||Cellular engineering of conduits for coronary and lower limb bypass surgery: Role of cell attachment peptides and pre-conditioning in optimising smooth muscle cells (SMC) adherence to compliant poly(carbonate-urea)urethane (MyoLink((TM))) scaffolds|
|Keywords:||cell adhesion, cell retention, compliance, FEPP plus, hybrid graft, polyurethanes, RGD, shear stress, smooth muscle cell, tissue engineering, PULSATILE SHEAR-STRESS, VASCULAR GRAFTS, IN-VITRO, BLOOD-VESSEL, POLYTETRAFLUOROETHYLENE GRAFTS, ENDOTHELIAL-CELLS, ADHESION PEPTIDES, REGENERATION PROCESS, ARTERIAL-WALL, RECONSTRUCTION|
|UCL classification:||UCL > School of Life and Medical Sciences > Faculty of Medical Sciences > Surgery and Interventional Science (Division of) > Research Department of General Surgery|
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