UCL logo

UCL Discovery

UCL home » Library Services » Electronic resources » UCL Discovery

Antibodies against homologous microbial caseinolytic proteases P characterise primary biliary cirrhosis

Bogdanos, DP; Baum, H; Sharma, UC; Grasso, A; Ma, Y; Burroughs, AK; Vergani, D; (2002) Antibodies against homologous microbial caseinolytic proteases P characterise primary biliary cirrhosis. J HEPATOL , 36 (1) 14 - 21.

Full text not available from this repository.

Abstract

Background/Aims: Antibodies to caseinolytic protease P177-194 (ClpP(177-194)) of the proteolytic subunit of the Clp complex of Escherichia coli (E. coli) are uniquely present in primary biliary cirrhosis (PBC). Molecular mimicry between the regulatory subunit ClpX and the principal T-cell epitope of pyruvate dehydrogenase complex ( PDC-E2) in PBC, has been proposed to account for this. Since ClpP is highly conserved among bacteria we investigated whether the micro-organisms triggering these antibodies may be other than E. coli.Methods/Results: E. Coli ClpP(177-194) is homologous with ClpP peptides of Yersinia enterocolitica (YEREN) and Haemophilus influenzae (HAEIN). Enzyme linked immunosorbent assay (ELISA) reactivity to these peptides was tested in 45 patients with PBC, 44 pathological and 32 healthy controls. Reactivity to at least one of the ClpP peptides was observed in 21 (47%) PBC patients, 5.8% pathological and 3.1% healthy controls (P < 0.01 for all). Among these 21 seropositive PBC patients, 15 (71%) reacted to ECOLI ClpP(177-194), alone or in association with YEREN and/or HAEIN peptides, compared to three (14.2%) reactive with YEREN, two (9.5%) with YEREN/HAEIN and one (4.7%) with HAEIN peptide. Simultaneous reactivity to homologous sequences was due to cross-reactivity as confirmed by competition ELISAs.Conclusions: The PBC-specificity of anti-microbial ClpP reactivity is confirmed: the questions as to primary trigger(s) and relevance to PBC pathogenesis remain open. (C) 2002 European Association for the Study of the Liver. Published by Elsevier Science B.V. All rights reserved.

Type: Article
Title: Antibodies against homologous microbial caseinolytic proteases P characterise primary biliary cirrhosis
Keywords: autoimmunity, cross-reactivity, infection, mimicry, YERSINIA-ENTEROCOLITICA INFECTION, 458 HOSPITALIZED-PATIENTS, PDC-E2 163-176 PEPTIDE, T-CELL EPITOPE, MOLECULAR MIMICRY, DIHYDROLIPOAMIDE ACETYLTRANSFERASE, DEHYDROGENASE COMPLEXES, AUTOIMMUNE-DISEASES, LIVER-DISEASE, UNITED-STATES
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine > Inst for Liver and Digestive Hlth
URI: http://discovery.ucl.ac.uk/id/eprint/119956
Downloads since deposit
0Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item