Tulley, PN; Neale, M; Jackson, D; Chana, JS; Grover, R; Cree, I; ... Wilson, GD; + view all Tulley, PN; Neale, M; Jackson, D; Chana, JS; Grover, R; Cree, I; Grobbelaar, AO; Wilson, GD; - view fewer (2004) The relation between c-myc expression and interferon sensitivity in uveal melanoma. BRIT J OPHTHALMOL , 88 (12) 1563 - 1567. 10.1136/bjo.2003.033498.
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Background/aim: Interferons (IFN) are currently being used to treat melanoma, including some patients with uveal melanoma. IFN is thought to inhibit tumour growth through downregulation of the c-myc oncogene; the overexpression of which has been shown to be associated with resistance in cell lines. The aim of this study was to investigate the relation between c-myc gene expression and IFN sensitivity in a series of uveal melanomas in a short term chemosensitivity assay.Methods: Tumours from 45 patients with uveal melanoma who had undergone enucleation were studied. The ATP chemosensitivity assay was used to study sensitivity to IFN-alpha-2b in freshly isolated cells from each tumour. Flow cytometry was used to assess c-myc expression in formalin fixed material from the primary specimens.Results: There was a wide range of IFN sensitivity between the specimens whereas c-myc expression was universal and present in 80% of the tumour cells in 80% of the specimens. Higher c-myc expression was associated with IFN-alpha resistance as measured by the maximum percentage of inhibition (p=0.05) and there was a trend with the IFN sensitivity index (p=0.07).Conclusions: These results demonstrate that tumours with high c-myc expression are also associated with IFN resistance. Future research is required to explore the potential of c-myc gene manipulation combined with IFN therapy.
|Title:||The relation between c-myc expression and interferon sensitivity in uveal melanoma|
|Keywords:||CELLS IN-VITRO, ONCOGENE EXPRESSION, CUTANEOUS MELANOMA, MALIGNANT-MELANOMA, PROGNOSTIC MARKER, HLA EXPRESSION, CLINICAL-SIGNIFICANCE, ADJUVANT THERAPY, OCULAR MELANOMA, DAUDI CELLS|
|UCL classification:||UCL > School of Life and Medical Sciences > Faculty of Medical Sciences > Surgery and Interventional Science (Division of)|
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