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Cloning and characterization of a novel human phosphatidylinositol transfer protein, rdgB beta

Fullwood, Y; dos Santos, M; Hsuan, JJ; (1999) Cloning and characterization of a novel human phosphatidylinositol transfer protein, rdgB beta. J BIOL CHEM , 274 (44) 31553 - 31558. Gold open access

Abstract

The various PITP, retinal degeneration B (rdgB), and amino-terminal domain interacting receptor (Nir) phosphatidylinositol transfer proteins can be divided into two structural families. The small, soluble PITP isoforms contain only a phosphatidylinositol transfer domain and have been implicated in phosphoinositide signaling and vesicle trafficking. In contrast, the rdgB proteins, which include Nir2 and Nir3, contain an amino-terminal PITP-like domain, an acidic, Ca2+-binding domain, six putative transmembrane domains, and a conserved carboxyl-terminal domain. However, the biological function of rdgB proteins is unclear, Here, we report the isolation of a cDNA encoding a novel rdgB protein, mammalian rdgB beta (MrdgB beta). The 38-kDa MrdgB beta protein contains an amino-terminal PITP-like domain and a short carboxyl-terminal domain. In contrast to other rdgB-like proteins, MrdgB beta contains no transmembrane motifs or the conserved carboxyl-terminal domain. Using Northern and reverse transcription-polymerase chain reaction analysis, we demonstrate that MrdgB beta mRNA is ubiquitously expressed. Immunofluorescence analysis of ectopic MrdgB beta showed cytoplasmic staining, and the ability of recombinant MrdgB beta to transfer phosphatidylinositol in vitro was similar to other PITP-like domains. Although early reports found functional degeneracy in, vitro, the identification of a fifth mammalian PITP-like protein with a unique domain organization and widespread expression supports more recent results that suggest that different PITP-like domains have distinct functions in vivo.

Type:Article
Title:Cloning and characterization of a novel human phosphatidylinositol transfer protein, rdgB beta
Open access status:An open access publication
Publisher version:http://www.jbc.org/content/early/recent/0
Keywords:RETINAL-DEGENERATION-B, CA2+-ACTIVATED SECRETION, DROSOPHILA-MELANOGASTER, GENE ENCODES, MUTANT, CELLS, PHOSPHORYLATION, MEMBRANE, HOMOLOG, ISOFORM

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