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Optimization of liposome mediated transfection of a neuronal cell line

McQuillin, A; Murray, KD; Etheridge, CJ; Stewart, L; Cooper, RG; Brett, PM; Miller, AD; (1997) Optimization of liposome mediated transfection of a neuronal cell line. NEUROREPORT , 8 (6) 1481 - 1484.

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Abstract

A cell line derived from sensory neurons was transfected with high efficiency using cationic liposomes, formulated from 3 beta[N-(N',N'-dimethylaminoethane)carbamoyl]cholesterol (DC-Chol) and dioleoyl L-alpha-phosphatidylethanolamine (DOPE). This is the first time that cationic liposomes of this type have been reported to transfect a neuronal cell line. We used a reporter gene construct expressing beta-galactosidase under the control of the cytomegalovirus immediate early promoter and routinely observed transfection efficiencies > 40%. Parameters affecting transfection efficiency were examined and the ratio of DNA to liposome proved to be crucial. Liposome formulation procedures and cell transfection protocols devised here will be used as a basis for further in vivo and in vitro work.

Type: Article
Title: Optimization of liposome mediated transfection of a neuronal cell line
Keywords: DC-Chol/DOPE, liposome, nerve, transfection, GENE-TRANSFER
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > Division of Psychiatry
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Eastman Dental Institute
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Eastman Dental Institute > Biomaterials and Tissue Eng
URI: http://discovery.ucl.ac.uk/id/eprint/118965
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