MAXWELL, PH and FERGUSON, DJP and OSMOND, MK and PUGH, CW and HERYET, A and DOE, BG and JOHNSON, MH and RATCLIFFE, PJ (1994) EXPRESSION OF A HOMOLOGOUSLY RECOMBINED ERYTHROPOIETIN-SV40 T-ANTIGEN FUSION GENE IN MOUSE-LIVER - EVIDENCE FOR ERYTHROPOIETIN PRODUCTION BY ITO CELLS. BLOOD , 84 (6) 1823 - 1830.
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Abstract
We have obtained transgenic mice in which an erythropoietin-SV40 virus T antigen fusion gene is homologously recombined into the native Epo locus. This gene is expressed in a tissue-specific manner closely resembling that of the native Epo gene. Immunohistochemical detection of SV40 T antigen has been used to characterize the hepatic cell populations expressing the transgene. In mice stimulated by anaemia or hypobaric hypoxia, SV40 T antigen was demonstrated in two liver cell populations: a subset of hepatocytes and a nonparenchymal cell type. Immunohistochemical and ultrastructural characterization of these cells by light and electron microscopy showed the nonparenchymal cell type to be the Ito cells, which lie in a persinusoidal position within the space of Disse. We therefore conclude that Ito cells are the nonhepatocytic source of liver Epo production. These cells show many similarities to the Epo-producing fibroblastoid interstitial cells of the kidney. (C) 1994 by The American Society of Hematology.
| Type: | Article |
|---|---|
| Title: | EXPRESSION OF A HOMOLOGOUSLY RECOMBINED ERYTHROPOIETIN-SV40 T-ANTIGEN FUSION GENE IN MOUSE-LIVER - EVIDENCE FOR ERYTHROPOIETIN PRODUCTION BY ITO CELLS |
| Keywords: | HEPATOCYTE GROWTH-FACTOR, FAT-STORING CELLS, INSITU HYBRIDIZATION, PERITUBULAR CELLS, TRANSGENIC MICE, MESSENGER-RNA, RAT-KIDNEY, IDENTIFICATION, LOCALIZATION, MACROPHAGE |
| UCL classification: | UCL > School of Life and Medical Sciences > Faculty of Medical Sciences > Medicine (Division of) |
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