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EXPRESSION OF A HOMOLOGOUSLY RECOMBINED ERYTHROPOIETIN-SV40 T-ANTIGEN FUSION GENE IN MOUSE-LIVER - EVIDENCE FOR ERYTHROPOIETIN PRODUCTION BY ITO CELLS

MAXWELL, PH; FERGUSON, DJP; OSMOND, MK; PUGH, CW; HERYET, A; DOE, BG; ... RATCLIFFE, PJ; + view all (1994) EXPRESSION OF A HOMOLOGOUSLY RECOMBINED ERYTHROPOIETIN-SV40 T-ANTIGEN FUSION GENE IN MOUSE-LIVER - EVIDENCE FOR ERYTHROPOIETIN PRODUCTION BY ITO CELLS. BLOOD , 84 (6) 1823 - 1830.

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Abstract

We have obtained transgenic mice in which an erythropoietin-SV40 virus T antigen fusion gene is homologously recombined into the native Epo locus. This gene is expressed in a tissue-specific manner closely resembling that of the native Epo gene. Immunohistochemical detection of SV40 T antigen has been used to characterize the hepatic cell populations expressing the transgene. In mice stimulated by anaemia or hypobaric hypoxia, SV40 T antigen was demonstrated in two liver cell populations: a subset of hepatocytes and a nonparenchymal cell type. Immunohistochemical and ultrastructural characterization of these cells by light and electron microscopy showed the nonparenchymal cell type to be the Ito cells, which lie in a persinusoidal position within the space of Disse. We therefore conclude that Ito cells are the nonhepatocytic source of liver Epo production. These cells show many similarities to the Epo-producing fibroblastoid interstitial cells of the kidney. (C) 1994 by The American Society of Hematology.

Type:Article
Title:EXPRESSION OF A HOMOLOGOUSLY RECOMBINED ERYTHROPOIETIN-SV40 T-ANTIGEN FUSION GENE IN MOUSE-LIVER - EVIDENCE FOR ERYTHROPOIETIN PRODUCTION BY ITO CELLS
Keywords:HEPATOCYTE GROWTH-FACTOR, FAT-STORING CELLS, INSITU HYBRIDIZATION, PERITUBULAR CELLS, TRANSGENIC MICE, MESSENGER-RNA, RAT-KIDNEY, IDENTIFICATION, LOCALIZATION, MACROPHAGE
UCL classification:UCL > School of Life and Medical Sciences > Faculty of Medical Sciences > Medicine (Division of)

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