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An acetylated form of histone H2A.Z regulates chromosome architecture in Schizosaccharomyces pombe

Kim, HS; Vanoosthuyse, V; Fillingham, J; Roguev, A; Watt, S; Kislinger, T; Treyer, A; ... Keogh, MC; + view all (2009) An acetylated form of histone H2A.Z regulates chromosome architecture in Schizosaccharomyces pombe. NAT STRUCT MOL BIOL , 16 (12) 1286 - U107. 10.1038/nsmb.1688.

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Abstract

Histone variant H2A.Z has a conserved role in genome stability, although it remains unclear how this is mediated. Here we demonstrate that the fission yeast Swr1 ATPase inserts H2A.Z (Pht1) into chromatin and Kat5 acetyltransferase (Mst1) acetylates it. Deletion or an unacetylatable mutation of Pht1 leads to genome instability, primarily caused by chromosome entanglement and breakage at anaphase. This leads to the loss of telomere-proximal markers, though telomere protection and repeat length are unaffected by the absence of Pht1. Strikingly, the chromosome entanglement in pht1 Delta anaphase cells can be rescued by forcing chromosome condensation before anaphase onset. We show that the condensin complex, required for the maintenance of anaphase chromosome condensation, prematurely dissociates from chromatin in the absence of Pht1. This and other findings suggest an important role for H2A.Z in the architecture of anaphase chromosomes.

Type: Article
Title: An acetylated form of histone H2A.Z regulates chromosome architecture in Schizosaccharomyces pombe
DOI: 10.1038/nsmb.1688
Keywords: CHROMATIN REMODELING COMPLEX, EARLY MAMMALIAN DEVELOPMENT, FISSION YEAST MITOSIS, SACCHAROMYCES-CEREVISIAE, VARIANT H2A.Z, PROTEIN, HETEROCHROMATIN, SEGREGATION, CONDENSIN, GENOME
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Genetics, Evolution and Environment
URI: http://discovery.ucl.ac.uk/id/eprint/118389
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