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CHARACTERIZATION OF 2 85KD PROTEINS THAT ASSOCIATE WITH RECEPTOR TYROSINE KINASES, MIDDLE-T/PP60C-SRC COMPLEXES, AND PI3-KINASE

OTSU, M; HILES, I; GOUT, I; FRY, MJ; RUIZLARREA, F; PANAYOTOU, G; ... WATERFIELD, MD; + view all (1991) CHARACTERIZATION OF 2 85KD PROTEINS THAT ASSOCIATE WITH RECEPTOR TYROSINE KINASES, MIDDLE-T/PP60C-SRC COMPLEXES, AND PI3-KINASE. CELL , 65 (1) 91 - 104.

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Abstract

Affinity-purified bovine brain phosphatidylinositol 3-kinase (PI3-kinase) contains two major proteins of 85 and 110 kd. Amino acid sequence analysis and cDNA cloning reveals two related 85 kd proteins (p85-alpha and p85-beta), which both contain one SH3 and two SH2 regions (src homology regions). When expressed, these 85 kd proteins bind to and are substrates for tyrosine-phosphorylated receptor kinases and the polyoma virus middle-T antigen/pp60c-src complex, but lack PI3-kinase activity. However, an antiserum raised against p85-beta immunoprecipitates PI3-kinase activity. The active PI3-kinase complex containing p85-alpha or p85-beta and the 110 kd protein binds to PDGF but not EGF receptors. p85-alpha and p85-beta may mediate specific PI3-kinase interactions with a subset of tyrosine kinases.

Type:Article
Title:CHARACTERIZATION OF 2 85KD PROTEINS THAT ASSOCIATE WITH RECEPTOR TYROSINE KINASES, MIDDLE-T/PP60C-SRC COMPLEXES, AND PI3-KINASE
Keywords:MIDDLE-T-ANTIGEN, GROWTH-FACTOR RECEPTOR, PHOSPHOLIPASE-C-II, PHOSPHATIDYLINOSITOL KINASE, PDGF RECEPTOR, SEQUENCE-ANALYSIS, MESSENGER-RNAS, BOVINE BRAIN, INOSITOL 3-PHOSPHATE, HEMATOPOIETIC-CELLS

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