UCL logo

UCL Discovery

UCL home » Library Services » Electronic resources » UCL Discovery

The response of osteoblasts to nanocrystalline silicon-substituted hydroxyapatite thin films

Thian, ES; Huang, J; Best, SM; Barber, ZH; Brooks, RA; Rushton, N; Bonfield, W; (2006) The response of osteoblasts to nanocrystalline silicon-substituted hydroxyapatite thin films. BIOMATERIALS , 27 (13) 2692 - 2698. 10.1016/j.biomaterials.2005.12.019.

Full text not available from this repository.

Abstract

Magnetron co-sputtering has been employed to fabricate thin nanocrystalline coatings of silicon-substituted hydroxyapatite (SiHA) of different Si compositions: 0.8 wt%, 2.2 wt%, and 4.9 wt%. A human osteoblast-like (HOB) cell model was used to study the long-term interaction between the HOB cells and coatings in vitro. Results showed that the number of cells growing on all coated titanium (Ti) samples were statistically significantly higher than on uncoated Ti. In addition, HOB cells growing on all SiHA surfaces displayed enhanced cell spreading, with extensive extracellular matrix synthesis. DNA staining revealed normal phenotype nuclear morphology for HOB cells, with several dense chromosomes surrounded by a periphery of intact nuclear membrane. Furthermore, immunofluorescent staining indicated that cells showed improved adhesion on the coated surfaces with increasing Si content, developing mature cytoskeletons with numerous distinct and well-defined actin stress fibres in the cell membranes. Results also demonstrated that the bone mineralisation process was greatest in the presence of the highest Si level (4.9 wt %). However, at very early culturing time point, cells did not attach so readily on the surface of this coating due to rapid dissolution. Thus, this work suggests that a Si content of 2.2 wt% may be the optimum loading to improve the bioactive property of HA thin films. (c) 2005 Elsevier Ltd. All rights reserved.

Type:Article
Title:The response of osteoblasts to nanocrystalline silicon-substituted hydroxyapatite thin films
DOI:10.1016/j.biomaterials.2005.12.019
Keywords:actin, biomineralisation, cell morphology, hydroxyapatite coating, osteoblast, silicon, IN-VITRO, BONE, REPLACEMENT, DEFICIENCY, COATINGS, ELEMENT, VIVO
UCL classification:UCL > School of BEAMS > Faculty of Engineering Science > Mechanical Engineering

Archive Staff Only: edit this record