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No further loss of dorsal root ganglion cells after axotomy in p75 neurotrophin receptor knockout mice

Sorensen, B; Tandrup, T; Koltzenburg, M; Jakobsen, J; (2003) No further loss of dorsal root ganglion cells after axotomy in p75 neurotrophin receptor knockout mice. J COMP NEUROL , 459 (3) 242 - 250. 10.1002/cne.10625.

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Abstract

The role of the p75 neurotrophin receptor for neuronal survival after nerve crush was studied in L5 dorsal root ganglia (DRG) of knockout mice and controls with assumption-free stereological methods. Numbers of neuronal A- and B-cells were obtained using the optical fractionator and optical disector techniques. At birth, the total number of DRG neurons was 10,000 +/- 2,600 in control mice compared with 5,100 +/- 1,300 in p75 knockout mice. During postnatal development, 1,400 neuronal B-cell bodies were lost in p75 knockouts (2P < 0.05) and 1,100 in controls (NS), whereas the A-cell population remained stable. After a sciatic nerve crush, the total neuron loss in controls was 15.4% +/- 3.5% (2P < 0.05) and 22.7% +/- 5.1% (2P < 0.05) at days 14 and 42, respectively. In contrast, there was no loss in total number of neurons after crush in p75 knockout mice. Neuronal A-cell number was unchanged after the crush in p75 knockouts as well as in controls at both times. At 14 days, the population of B-cells was reduced by 24.8% +/- 3.6% in controls and by 6.1% +/- 3.5% in p75 knockouts, this difference being significant (2P < 0.001). At 42 days, the B-cell loss was 29.6% +/- 5.5% in controls and 4.2% +/- 6.4% in p75 knockouts (2P < 0.001). In conclusion, the lack of the p75 receptor results in neuronal DRG cells that are resistant to nerve injury, pointing to a role for the receptor in apoptosis. (C) 2003 Wiley-Liss, Inc.

Type: Article
Title: No further loss of dorsal root ganglion cells after axotomy in p75 neurotrophin receptor knockout mice
DOI: 10.1002/cne.10625
Keywords: axotomy, neuronal survival, regeneration, stereology, NERVE GROWTH-FACTOR, FOREBRAIN CHOLINERGIC NEURONS, AFFINITY NGF RECEPTOR, SENSORY NEURONS, SCIATIC-NERVE, POSTNATAL-DEVELOPMENT, ARBITRARY PARTICLES, MOLECULAR-CLONING, GENE-TRANSFER, TOTAL NUMBER
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology > Clinical and Movement Neurosciences
URI: http://discovery.ucl.ac.uk/id/eprint/116354
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