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Organization, structure and alternate splicing of the murine RFC-1 gene encoding a folate transporter

Tolner, B; Roy, K; Sirotnak, FM; (1997) Organization, structure and alternate splicing of the murine RFC-1 gene encoding a folate transporter. GENE , 189 (1) 1 - 7.

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Abstract

The structural organization of the murine RFC-1 gene encoding a folate transporter has been determined. The entire nucleotide sequence of the L1210 cell RFC-1 cDNA, the 3'- and 5'-untranslated regions and the coding sequence were found to be distributed in eight exons, including six primary exons and alternates to exon 1 and exon 5, spanning 10.4 kb. Splice variants were identified in an L1210 cell cDNA library. The most common incorporates exons 1 through 6, encoding a 58-kDa polypeptide. The two least common incorporate exons 1 and 2, a truncated version of exon 3 and exons 4 through 6; or exons 1 through 4, an alternate to exon 5, and exon 6, encoding polypeptides of 53.6 and 43.4 kDa, respectively. A fourth variant reported earlier (GenBank/EMBL accession No. L36539) by others incorporates what we have found to be an alternate of exon 1 and exons 2 through 6. A relatively GC-rich region of the genome just 5' of exon 1 as well as exon la appears to be distinctly promoter-like and encodes a number of putative cis-acting elements. The findings pertaining to alternates of exon I suggest that the transcription of RFC-1 variants results from two different promoters. (C) 1997 Elsevier Science B.V.

Type: Article
Title: Organization, structure and alternate splicing of the murine RFC-1 gene encoding a folate transporter
Keywords: murine RFC-1 gene, folate transport, LEUKEMIA-CELL LINE, HUMAN TUMOR-CELLS, ESCHERICHIA-COLI, METHOTREXATE SENSITIVITY, MEMBRANE CARRIER, L1210 CELL, EXPRESSION, PROTEIN, VARIANTS, CDNA
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute > Research Department of Oncology
URI: http://discovery.ucl.ac.uk/id/eprint/114660
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