Fitzhenry, RJ and Reece, S and Trabulsi, LR and Heuschkel, R and Murch, S and Thomson, A and Frankel, G and Phillips, AD (2002) Tissue tropism of enteropathogenic Escherichia coli strains belonging to the O55 serogroup. Infection and Immunity , 70 (8) 4362 - 4368.
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Four enteropathogenic Escherichia coli (EPEC) strains belonging to the 055 serogroup (G21 and G30 [both 055:H6], G35 [055:H-], and G58 [055:H7]) were tested for their tissue tropism by using human intestinal in vitro organ culture. Strains showed restricted adhesion with attaching-and-effacing activity to follicle-associated epithelium of Peyer's patches, with no apparent adhesion to duodenum or colon. G35 and G58 express intimin gamma and show a similar tropism to intimin gamma- expressing enterohemorrhagic E. coli (EHEC) 0157:H7. However, strains G21 and G30 were unusual because they expressed intimin (x and had a restricted tissue tropism of intimin gamma phenotype. The amino acid sequence of the carboxy-terminal 280 amino acids of intimin from G21 was determined. Comparison with the prototype intimin alpha from strain E2348/69 (0127:H6) showed a single amino acid difference (corresponding to Val907 and Ala907 in the whole intimins). This mutation was reproduced by site-directed mutagenesis in an intimin (x plasmid template, pCVD438, with the hypothesis that it may induce a change in tropism. However, when the mutated plasmid was placed in both EPEC and EHEC backgrounds, duodenal adhesion in a manner similar to strain E2348/69 was evident upon in vitro organ culture. Thus, additional factor(s) unrelated to intimin exist in the 055:116 genome that influence human intestinal tissue tropism
|Title:||Tissue tropism of enteropathogenic Escherichia coli strains belonging to the O55 serogroup|
|Additional information:||Journal English Article AMER SOC MICROBIOLOGY AUG 574VC WASHINGTON INFEC IMMUNITY 1752 N ST NW, WASHINGTON, DC 20036-2904 USA|
|Keywords:||ACID, Acids, ACTIN CONDENSATION, activity, additional, Adhesion, ALPHA, amino acid, Amino Acid Sequence, Amino Acids, AMINO-ACID-SEQUENCE, Brazil, Colon, comparison, culture, difference, duodenal, Duodenum, E, EAEA GENE, EFFACING PHENOTYPE, EHEC, English, ENTEROCYTE EFFACEMENT, enteropathogenic, EPEC, EPITHELIA, Epithelium, Escherichia coli, ESCHERICHIA-COLI, gamma, Genome, HYPOTHESIS, Immunity, in vitro, In-vitro, INDUCE, Infection, intestinal, intimin, INTIMIN-ALPHA, LOCUS, May, microbiology, Mutagenesis, Mutation, N, Organ Culture, ORGAN-CULTURE, PATHOGENS, Peyer's Patches, Phenotype, plasmid, Proteins, Receptor, SEQUENCE, SINGLE, site-directed mutagenesis, ST, Strain, STRAINS, Tissue, Tropism, USA, VITRO|
|UCL classification:||UCL > School of Life and Medical Sciences > Faculty of Population Health Sciences > Institute of Child Health|
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