Binding of Clostridium difficile surface layer proteins to gastrointestinal tissues.
Infection and Immunity
5770 - 5778.
Clostridium difficile is the etiological agent of antibiotic- associated diarrhea, a potentially serious condition frequently affecting elderly hospitalized patients. While tissue damage is primarily induced by two toxins, the mechanism of gut colonization, and particularly the role of bacterial adherence to the mucosa, remains to be clarified. Previous studies have shown binding of C. difficile whole cells to cultured cell lines and suggested the existence of multiple adhesins, only one of which has been molecularly characterized. In this paper, we have investigated tissue binding of C. difficile surface layer proteins (SLPs), which are the predominant outer surface components and are encoded by the slpA gene. The adherence of C. difficile to HEp-2 cells was studied by enzyme-linked immunosorbent assay and fluorescence-activated cell sorter analysis, which showed that antibodies to the high-molecular- weight (MW) SLP inhibited adherence. Immunohistochemical analysis of human gastrointestinal tissue sections revealed strong binding both to the surface epithelium lining the digestive cavities and to the subjacent lamina propria, while glands were negative. A similar pattern was observed in the mouse. By using purified recombinant SLPs, we show that binding is largely mediated by the high-MW SLP. By Western blotting analysis, we have identified two potential ligands of the C. difficile SLPs, one of which may be specific to the gut. By using purified extracellular matrix components immobilized on nitrocellulose, we also show SLP binding to collagen I, thrombospondin, and vitronectin, but not to collagen IV, fibronectin, or laminin. These results raise the possibility that the SLPs play a role both in the initial colonization of the gut by C. difficile and in the subsequent inflammatory reaction
|Title:||Binding of Clostridium difficile surface layer proteins to gastrointestinal tissues|
|Additional information:||Journal English Article AMER SOC MICROBIOLOGY OCT 595TU WASHINGTON INFEC IMMUNITY 1752 N ST NW, WASHINGTON, DC 20036-2904 USA|
|Keywords:||adherence, AGENT, analysis, antibiotic, antibodies, Antibody, ASSAY, BACTERIAL, BINDING, Cavity, cell, Cell Line, Cell lines, CELL-LINE, CELL-LINES, CELLS, Child, Clostridium difficile, Collagen, COLLAGEN ADHESIN, COLONIZATION, COMPONENT, COMPONENTS, Condition, CONTROLLED GENE-EXPRESSION, Damage, DIARRHEA, elderly, English, enzyme linked immunosorbent assay, Enzyme-Linked Immunosorbent Assay, EPITHELIA, Epithelium, Extracellular Matrix, EXTRACELLULAR-MATRIX, Fibronectin, Gastrointestinal, GENE, GNOTOBIOTIC MICE, Gut, HOSPITALIZED-PATIENTS, Immunity, Immunohistochemical analysis, IV, LAMINA PROPRIA, LAMININ, layer, LIGAND, ligands, LINE, LINES, LISTERIA- MONOCYTOGENES, MATRIX, May, MECHANISM, microbiology, mouse, MUCOSA, multiple, N, PAPER, Patient, patients, Pattern, play, PROTEIN, Proteins, Receptor, recombinant, Result, SECTIONS, ST, STAPHYLOCOCCUS-AUREUS, SUBSEQUENT, SURFACE, THROMBOSPONDIN-1, Tissue, Tissue Section, TISSUE-SECTIONS, Tissues, TOXIN, Toxins, USA, Vaccination, Vitronectin, WEIGHT, western blotting, YERSINIA|
|UCL classification:||UCL > School of Life and Medical Sciences > Faculty of Population Health Sciences > Institute of Child Health|
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