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The expression profile for the tumour suppressor gene PTEN and associated polymorphic markers.
British Journal of Cancer
1671 - 1676.
PTEN, a putative tumour suppressor gene associated with prostate and other cancers, is known to be located within the chromosomal region 10q23.3. Transcription of the PTEN gives rise to multiple mRNA species. Analyses by Northern blots, using cell lines which express PTEN together with cell lines which have lost the PTEN or carry a truncated version of the gene, has allowed us to demonstrate that the pseudogene is not transcribed. In addition, 3' RACE studies confirmed that the multiple mRNA species arising from the gene probably result from the use of alternative polyadenylation sites. No evidence for tissue- or cell-specific patterns of transcription was found. Analysis by 5' RACE placed the putative site for the start of transcription around 830 bp upstream of the start codon. A map of the location of the PTEN gene with a series of overlapping YAC, BAC and PACs has been constructed acid the relative position of eight microsatellite markers sited. Two known and one novel marker have been positioned within the gene, the others are in flanking regions. The more accurate location of these markers should help in future studies of the extent of gene loss. Several polymorphisms were also identified, all were within introns. Four of the common polymorphisms appear to be linked. In blood, DNA from 200 individuals, including normal, BPH and prostate cancer patients, confirmed this link. Only two samples of 200 did not carry the linked haplotype, both were patients with advanced prostate cancer. It is possible that such rearrangements within PTEN could be evidence of predisposition to prostate cancer in this small number of cases. (C) 2000 Cancer Research Campaign
|Title:||The expression profile for the tumour suppressor gene PTEN and associated polymorphic markers|
|Additional information:||WoS ID: 000086906400010 Article|
|Keywords:||Chromosomes, expression, GENE, Genes, Heterozygosity, Suppressor, Tumour, ACID, analysis, Blood, cancer, CANCER PATIENTS, CANCER-PATIENTS, cancers, cell, Cell Line, Cell lines, CELL-LINE, CODON, COMMON, DNA, EXTENT, FOCAL ADHESION KINASE, future, GERMLINE MUTATIONS, GRADE GLIOMAS, INACTIVATION, individuals, Introns, link, LOCATION, MICROSATELLITE, microsatellite markers, mRNA, NO EVIDENCE, novel, NUMBER, Other, Patient, patients, Pattern, PATTERNS, phosphatase, Polymorphism, polymorphisms, POSITION, prostate, Prostate Cancer, PTEN, PTEN/MMAC1, REARRANGEMENT, REGION, regions, REGULATORY ROLE, Research, SAMPLE, SERIES, SITE, Tissue, TRANSCRIPTION, US|
|UCL classification:||UCL > School of Life and Medical Sciences > Faculty of Medical Sciences > Medicine (Division of)
UCL > School of Life and Medical Sciences > Faculty of Medical Sciences > Surgery and Interventional Science (Division of)
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