UCL logo

UCL Discovery

UCL home » Library Services » Electronic resources » UCL Discovery

In vitro isoflavone supplementation reduces hydrogen peroxide-induced DNA damage in sperm

Sierens, J; Hartley, JA; Campbell, MJ; Leathem, AJC; Woodside, JV; (2002) In vitro isoflavone supplementation reduces hydrogen peroxide-induced DNA damage in sperm. TERATOGEN CARCIN MUT , 22 (3) 227 - 234. 10.1002/tcm.10015.

Full text not available from this repository.

Abstract

Isoflavones are plant compounds, proposed to have health benefits in a variety of human diseases, including coronary heart disease and endocrine-responsive cancers. Their physiological effects include possible antioxidant activity, therefore suggesting a role for isoflavones in the prevention of male infertility. The aim of this study was to test the antioxidant effects of the isoflavones genistein and equol on sperm DNA integrity, assessed in vitro after hydrogen peroxide-mediated damage, using the cornet assay. Pre-treatment with genistein or equol at doses of 0.01-100 mumol/l significantly protected sperm DNA against oxidative damage. Both ascorbic acid (10-600 mumol/l) and alpha-tocopherol (1-100 mumol/l) also protected. Compared with ascorbic acid and alpha-tocopherol, added at physiological concentrations, genistein was the most potent antioxidant, followed by equol, ascorbic acid, and alpha-tocopherol. Genistein and equol added in combination were more protective than when added singly. Based on these preliminary data, which are similar to those observed previously in lymphocytes, these compounds may have a role to play in antioxidant protection against male infertility.

Type: Article
Title: In vitro isoflavone supplementation reduces hydrogen peroxide-induced DNA damage in sperm
DOI: 10.1002/tcm.10015
Keywords: antioxidants, DNA damage, infertility, isoflavones, sperm, HUMAN-SPERMATOZOA, ANTIOXIDANT SUPPLEMENTATION, LIPID-PEROXIDATION, MALE-INFERTILITY, SEMINAL PLASMA, DOUBLE-BLIND, PHYTOESTROGEN, CELLS, MEN, FRAGMENTATION
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute > Research Department of Oncology
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Pop Health Sciences > Inst for Women's Health
URI: http://discovery.ucl.ac.uk/id/eprint/108992
Downloads since deposit
0Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item