UCL logo

UCL Discovery

UCL home » Library Services » Electronic resources » UCL Discovery

Development of a didanosene genotypic resistance interpretation system based on large derivation and validation datasets

Assoumou, L; Cozzi-Lepri, A; Brun-Vezinet, F; DeGruttola, V; Kuritzkes, DR; Phillips, A; Zolopa, A; ... Forum Collaborative HIV Res, ; + view all (2010) Development of a didanosene genotypic resistance interpretation system based on large derivation and validation datasets. AIDS , 24 (3) 365 - 371. 10.1097/QAD.0b013e32833338ba.

Full text not available from this repository.

Abstract

Objective: To assess the genotypic determinants of the virological response to didanosine (ddI) in HIV-infected patients.Methods: The Forum database on ddI was randomly divided into a derivation set (n=1000) and a validation set (n=453). Linear regression models and bootstrap sampling were used to select resistance mutations and to estimate their resistance scores. Linear regression models, accounted for the censoring of viral load measurements due to assay lower limits, of the week 8 reduction in viral load from baseline were adjusted for baseline viral load, the exact number of weeks between baseline and the week 8 viral load measurements, and the Stanford genotypic sensitivity score.Results: The ddI resistance mutations and their resistance scores based on the derivation set were as follows: M41L (score of 14), T69D (24), D123S (40), T139M (54), I180V (53), M184V (-12), V1891 (55), Q207K (37), L210W (25), and T215Y (eight). The total score is obtained by adding the individual scores. Viruses with scores of 19 or less, 20-59, and 60 or more are considered sensitive, intermediate, and resistant, respectively. In the validation set, respectively, 58.7, 36.9, and 4.4% of viruses were predicted to be sensitive, intermediate, and resistant to ddI. The observed viral load reductions at week 8 were, respectively, 1.51 log(10)copies/ml [interquartile range (IQR) 1.26-1.76] (P=0.0001 versus resistant), 1.11 (0.94-1.30) (P=0.0077 versus sensitive), and 0.46 (0.32-0.74) (P=0.0079 versus intermediate).Conclusion: We developed a genotypic resistance score for didanosine including four mutations never previously used. (C) 2010 Wolters Kluwer Health vertical bar Lippincott Williams & Wilkins

Type: Article
Title: Development of a didanosene genotypic resistance interpretation system based on large derivation and validation datasets
Location: Sitges, SPAIN
DOI: 10.1097/QAD.0b013e32833338ba
Keywords: didanosine algorithm, didanosine genotypic resistance score, didanosine interpretation system, didanosine scoring system, didanosine, genotypic resistance score, viral load, IMMUNODEFICIENCY-VIRUS TYPE-1, HIV-1 REVERSE-TRANSCRIPTASE, VIROLOGICAL RESPONSE, HIV-1-INFECTED PATIENTS, MUTATION
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Pop Health Sciences > Institute for Global Health
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Pop Health Sciences > Institute for Global Health > Infection and Population Health
URI: http://discovery.ucl.ac.uk/id/eprint/106742
Downloads since deposit
0Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item