HELLER, C and BECK, S (1992) FIELD INVERSION GEL-ELECTROPHORESIS IN DENATURING POLYACRYLAMIDE GELS. NUCLEIC ACIDS RES , 20 (10) 2447 - 2452.
The velocities of single stranded DNA molecules in denaturing polyacrylamide gels during symmetric and asymmetric field inversion were measured at different pulse times and gel concentrations. Under the conditions chosen in our study, pulse times as short as a few milliseconds lead to a retardation of DNA molecules larger than 400 bases. We found that a field inversion with an electric field in the forward direction of about double the strength of that applied in the backward direction is a good compromise between the degree of retardation, the temperature control requirements and the run time of the gel.
|Title:||FIELD INVERSION GEL-ELECTROPHORESIS IN DENATURING POLYACRYLAMIDE GELS|
|Open access status:||An open access publication|
|Keywords:||50-KILOBASE DNA FRAGMENTS, ELECTRIC-FIELD, SEPARATING 1-KILOBASE, SEQUENCING GELS, MOLECULES, RESOLUTION, REPTATION, MOBILITY|
|UCL classification:||UCL > School of Life and Medical Sciences > Faculty of Medical Sciences > Wolfson Institute and Cancer Institute Administration > Cancer Institute > Research Department of Cancer Biology|
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