UCL logo

UCL Discovery

UCL home » Library Services » Electronic resources » UCL Discovery

Replication of mitochondrial DNA occurs throughout the mitochondria of cultured human cells

Magnusson, J; Orth, M; Lestienne, P; Taanman, JW; (2003) Replication of mitochondrial DNA occurs throughout the mitochondria of cultured human cells. EXP CELL RES , 289 (1) 133 - 142. 10.1016/S0014-4827(03)00249-0.

Full text not available from this repository.

Abstract

Replication of mitochondrial DNA (mtDNA) is dependent on nuclear-encoded factors. It has been proposed that this reliance may exert spatial restrictions on the sites of mtDNA replication within the cytoplasm, as a previous study only detected mtDNA synthesis in perinuclear mitochondria. We have studied mtDNA replication in situ in a variety of human cell cultures labeled with 5-bromo-2'-deoxyuridine. In contrast to what has been reported, mtDNA synthesis was detected at multiple sites throughout the mitochondrial network following short pulses with bromodeoxyuridine. Although no bromodeoxyuridine incorporation was observed in anuclear platelets, incorporation into mtDNA of fibroblasts that had been enucleated 2 h prior to labeling was readily detectable. Blotting experiments indicated that the bromodeoxyuridine incorporation into mtDNA observed in situ represents replication of the entire mtDNA molecule. The studies also showed that replication of mtDNA occurred at any stage of the cell cycle in proliferating cells and continued in postmitotic cells, although at a lower level. These results demonstrate that mtDNA replication is not restricted to mitochondria in the proximity of the nucleus and imply that all components of the replication machinery are available at sufficient levels throughout the mitochondrial network to permit mtDNA replication throughout the cytoplasm. (C) 2003 Elsevier Science (USA). All rights reserved.

Type: Article
Title: Replication of mitochondrial DNA occurs throughout the mitochondria of cultured human cells
DOI: 10.1016/S0014-4827(03)00249-0
Keywords: mitochondria, mitochondrial DNA, replication, 5-bromo-2 '-deoxyuridine, cell cycle, DNA polymerase gamma, mitochondrial transcription factor A, TRANSCRIPTION FACTOR-A, POLYMERASE-GAMMA, ACCESSORY SUBUNIT, MTDNA, DEPLETION, MAINTENANCE, MOLECULES, MECHANISM, SEQUENCE, PROTEIN
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology > Clinical and Movement Neurosciences
URI: http://discovery.ucl.ac.uk/id/eprint/104574
Downloads since deposit
0Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item