UCL logo

UCL Discovery

UCL home » Library Services » Electronic resources » UCL Discovery

Molecular cloning of CoA synthase - The missing link in CoA biosynthesis

Zhyvoloup, A; Nemazanyy, I; Babich, A; Panasyuk, G; Pobigailo, N; Vudmaska, M; Naidenov, V; ... Gout, IT; + view all (2002) Molecular cloning of CoA synthase - The missing link in CoA biosynthesis. J BIOL CHEM , 277 (25) 22107 - 22110. 10.1074/jbc.C200195200. Gold open access

Abstract

Coenzyme A functions as a carrier of acetyl and acyl groups in living cells and is essential for numerous biosynthetic, energy-yielding, and degradative metabolic pathways. There are five enzymatic steps in CoA biosynthesis. To date, molecular cloning of enzymes involved in the CoA biosynthetic pathway in mammals has been only reported for pantothenate kinase. In this study, we present cDNA cloning and functional characterization of CoA synthase. It has an open reading frame of 563 aa and encodes a protein of similar to60 kDa. Sequence alignments suggested that the protein possesses both phosphopantetheine adenylyltransferase and dephospho-CoA kinase domains. Biochemical assays using wild type recombinant protein confirmed the gene product indeed contained both these enzymatic activities. The presence of intrinsic phosphopantetheine adenylyltransferase activity was further confirmed by site-directed mutagenesis. Therefore, this study describes the first cloning and characterization of a mammalian CoA synthase and confirms this is a bifunctional enzyme containing the last two components of CoA biosynthesis.

Type: Article
Title: Molecular cloning of CoA synthase - The missing link in CoA biosynthesis
Open access status: An open access publication
DOI: 10.1074/jbc.C200195200
Keywords: PANTOTHENATE KINASE GENE, COENZYME-A METABOLISM, ESCHERICHIA-COLI, RAT-LIVER, ADENYLYLTRANSFERASE, HEART, PURIFICATION, COMPLEX, MUSCLE, STATE
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Structural and Molecular Biology
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute > Research Department of Oncology
URI: http://discovery.ucl.ac.uk/id/eprint/10445
Downloads since deposit
0Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item