UCL logo

UCL Discovery

UCL home » Library Services » Electronic resources » UCL Discovery

Inhibition of T-cell response by Escherichia coli heat-labile enterotoxin-treated epithelial cells

Lopes, LM; Maroof, A; Dougan, G; Chain, BM; (2000) Inhibition of T-cell response by Escherichia coli heat-labile enterotoxin-treated epithelial cells. INFECT IMMUN , 68 (12) 6891 - 6895.

Full text not available from this repository.

Abstract

Escherichia coli heat-labile enterotoxin (LT) is an extensively studied adjuvant of mucosal responses. Nevertheless, its mode of action as an adjuvant remains incompletely understood. In this study, we describe a simplified in vitro model with which to look at some aspects of immunoregulation by LT. The interaction of LT with the apical surface of a monolayer of CaCo-2 epithelial cells induces the release of a soluble factor which inhibits the antigen-induced release of interleukin-2 by T cells cultured at the basolateral side of the cells. The release of this factor requires the ADP-ribosylating activity of LT since the isolated B subunit, as well as an enzymatically silent LT mutant, loses biological activity in this model. The inhibitory activity is likely to be due to prostaglandin release, since it is blocked by indomethacin. The contribution of LT-induced prostaglandin release to the complex immunoregulatory activity of LT is discussed.

Type: Article
Title: Inhibition of T-cell response by Escherichia coli heat-labile enterotoxin-treated epithelial cells
Keywords: CHOLERA-TOXIN, MUCOSAL ADJUVANTICITY, B-SUBUNIT, RECEPTOR, MUTANT, MODULATION, INDUCTION, IL-12, CAMP
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Computer Science
URI: http://discovery.ucl.ac.uk/id/eprint/103388
Downloads since deposit
0Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item