Stonehouse, TJ and Woodhead, VE and Herridge, PS and Ashrafian, H and George, M and Chain, BM and Katz, DR (1999) Molecular characterization of U937-dependent T-cell co-stimulation. IMMUNOLOGY , 96 (1) 35 - 47.
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U937 cells provide a co-stimulatory signal for CD3-mediated T-cell activation which is independent of the CD28/CD80/CD86 interaction. This study set out to identify which molecules contribute to this co-stimulatory activity. Monoclonal antibodies (mAb) to the known accessory molecules CD11a, CD18, CD54 and CD45, all inhibited T-cell proliferation. Although CD11a/18 mAb inhibited U937/T-cell cluster formation as well as proliferation, CD45 enhanced the size of the clusters formed, suggesting that this was not the only mechanism of inhibition. The alternative co-stimulatory pathway provided by U937 cells preferentially stimulated a response in the CD18(+) T-cell population, and this reflected the reduced sensitivity of CD8(+) T cells to CD28-mediated activation. Monoclonal antibodies to three molecules, CD53, CD98 and CD147, also inhibited U937-dependent T-cell proliferation. The mAb to CD98 and CD147 were inhibitory when prepulsed on to the U937 cells, suggesting an effect mediated by these molecules on the antigen-presenting cell.
|Title:||Molecular characterization of U937-dependent T-cell co-stimulation|
|Keywords:||PROTEIN-TYROSINE KINASE, BLOOD-BRAIN-BARRIER, COSTIMULATORY SIGNAL, SURFACE-PROTEINS, ANTIGEN RECEPTOR, ACTIVATION, CD28, ASSOCIATION, PROLIFERATION, PHOSPHATASE|
|UCL classification:||UCL > School of Life and Medical Sciences > Faculty of Medical Sciences > Infection and Immunity (Division of) > Research Department of Immunology|
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