UCL logo

UCL Discovery

UCL home » Library Services » Electronic resources » UCL Discovery

SENSITIZATION OF ALLO-SPECIFIC T-LYMPHOCYTES INVIVO - ROLE OF ANTIGEN-PRESENTING CELLS

IBRAHIM, MAA; ELLIS, JS; CHAIN, BM; KATZ, DR; (1992) SENSITIZATION OF ALLO-SPECIFIC T-LYMPHOCYTES INVIVO - ROLE OF ANTIGEN-PRESENTING CELLS. IMMUNOBIOLOGY , 186 (5) 362 - 377.

Full text not available from this repository.

Abstract

The migratory behavior of antigen-presenting cells was investigated in vivo. Purified murine splenic dendritic cells and splenic and peritoneal macrophages were labelled and injected subcutaneously in the hind foot-pads of mice and monitored for seven days. In, the first 24 h, a small quantity of label was recovered from popliteal but not inguinal lymph nodes with radioactive (In-111-oxine and H-3-uridine) but not fluorescent (1,1'-dioctadecyl 3,3,3',3'-tetra-methylindocarbocyanine perchlorate and fluorescein isothiocyanate) labelling of the antigen-presenting cells. Chemical fixation of the injected antigen-presenting cells had no effect on the detection of label in the popliteal lymph nodes, suggesting that it was unlikely to be due to active cellular migration. Label recovery from hind feet declined with time over the seven day period and was independent of the label type. Essentially the same observations were made whether the antigen-presenting cells were syngeneic or allogeneic to the injected mice and irrespective of the type of antigen-presenting cell used. However, allogeneic antigen-presenting cells, which did not migrate to the draining lymph nodes, successfully primed T lymphocytes in these lymph nodes as shown by a secondary in vitro mixed leukocyte reaction. Again, chemical fixation of the injected antigen-presenting cells had no effect on their ability to prime allogeneic T lymphocytes in the draining lymph nodes. These experiments suggest that, during experimental allo-sensitization via die subcutaneous route, indirect priming of allogeneic T lymphocytes may be a dominant pathway.

Type: Article
Title: SENSITIZATION OF ALLO-SPECIFIC T-LYMPHOCYTES INVIVO - ROLE OF ANTIGEN-PRESENTING CELLS
Keywords: MINOR H-ANTIGENS, DENDRITIC CELLS, MONOCLONAL-ANTIBODY, LANGERHANS CELLS, CROSS-REACT, LYMPH-NODES, ALLOGRAFTS, MIGRATION, MOUSE, ISOTHIOCYANATE
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Infection and Immunity
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Computer Science
URI: http://discovery.ucl.ac.uk/id/eprint/103326
Downloads since deposit
0Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item