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Predicting metal-binding site residues in low-resolution structural models

Sodhi, JS; Bryson, K; McGuffin, LJ; Ward, JJ; Wernisch, L; Jones, DT; (2004) Predicting metal-binding site residues in low-resolution structural models. J MOL BIOL , 342 (1) 307 - 320. 10.1016/j.jmb.2004.07.019.

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Abstract

The accurate prediction of the biochemical function of a protein is becoming increasingly important, given the unprecedented growth of both structural and sequence databanks. Consequently, computational methods are required to analyse such data in an automated manner to ensure genomes are annotated accurately. Protein structure prediction methods, for example, are capable of generating approximate structural models on a genome-wide scale. However, the detection of functionally important regions in such crude models, as well as structural genomics targets, remains an extremely important problem. The method described in the current study, MetSite, represents a fully automatic approach for the detection of metal-binding residue clusters applicable to protein models of moderate quality The method involves using sequence profile information in combination with approximate structural data. Several neural network classifiers are shown to be able to distinguish metal sites from non-sites with a mean accuracy of 94.5%.The method was demonstrated to identify metal-binding sites correctly in LiveBench targets where no obvious metal-binding sequence motifs were detectable using InterPro. Accurate detection of metal sites was shown to be feasible for low-resolution predicted structures generated using mGenTHREADER where no side-chain information was available. High-scoring predictions were observed for a recently solved hypothetical protein from Haemophilus influenzae, indicating a putative metal-binding site. (C) 2004 Elsevier Ltd. All rights reserved.

Type:Article
Title:Predicting metal-binding site residues in low-resolution structural models
DOI:10.1016/j.jmb.2004.07.019
Keywords:functional site, structural genomics, PSSM, metal binding, model proteins, GENOMIC THREADING DATABASE, PROTEIN STRUCTURES, FOLD RECOGNITION, FUNCTIONAL SITES, FAMILIES, CLASSIFICATION, GENTHREADER, SEQUENCES, RESOURCE, SERVERS
UCL classification:UCL > School of BEAMS > Faculty of Engineering Science > Computer Science

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