UCL logo

UCL Discovery

UCL home » Library Services » Electronic resources » UCL Discovery

Bluetongue virus entry into cells.

Forzan, M; Marsh, M; Roy, P; (2007) Bluetongue virus entry into cells. J Virol , 81 (9) pp. 4819-4827. 10.1128/JVI.02284-06.

Full text not available from this repository.


Bluetongue virus (BTV) is a member of the Orbivirus genus within the Reoviridae family. Like those of other members of the family, BTV particles are nonenveloped and contain two distinct capsids, namely, an outer capsid and an inner capsid or core. The two outer capsid proteins, VP2 and VP5, are involved in BTV entry into cells and in the delivery of the transcriptionally active core to the target cell cytoplasm. However, very little is known about the precise mechanism of BTV entry. In this report, using RNA interference, we demonstrate that inhibition of the clathrin-dependent endocytic pathway correlates with reduced BTV internalization and subsequent replication. Furthermore, by using the ATPase inhibitor bafilomycin A1, we show that exposure of the virus to acidic pH is required for productive infection. Moreover, microscopic analysis of cells incubated with BTV indicated that the virus is internalized into early endosomes, where separation of the outer capsid and inner core occurs. Together, our data indicate that BTV undergoes low-pH-induced penetration in early endosomes following clathrin-mediated endocytosis from the plasma membrane, supporting a stepwise model for BTV entry and penetration.

Type: Article
Title: Bluetongue virus entry into cells.
Location: United States
DOI: 10.1128/JVI.02284-06
Keywords: Animals, Bluetongue virus, Cell Membrane, HeLa Cells, Humans, Hydrogen-Ion Concentration, Immunoblotting, Macrolides, Microscopy, Confocal, Models, Biological, RNA Interference, Transport Vesicles, Virus Internalization
UCL classification: UCL > School of Life and Medical Sciences
UCL > School of Life and Medical Sciences > Faculty of Life Sciences
URI: http://discovery.ucl.ac.uk/id/eprint/102325
Downloads since deposit
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item