Differential involvement of beta(3) integrin in pre- and postsynaptic forms of adaptation to chronic activity deprivation.
NEURON GLIA BIOL
179 - 187.
Neuronal networks can adapt to global changes in activity levels through compensatory modifications in pre- and postsynaptic parameters of synaptic transmission. These forms of synaptic plasticity are known as synaptic homeostasis, and are thought to require specific cellular interactions and signaling across the entire neuronal network. However, the molecular mechanisms underlying synaptic homeostasis have so far been investigated mostly in primary cultures of dissociated neurons, a preparation that lacks the specificity of in vivo circuitry. Here, we show that there are critical differences in the properties of synaptic homeostasis between dissociated neuronal cultures and organotypic slices, a preparation that preserves more precisely in vivo connectivity. Moreover, the cell adhesion molecule beta(3) integrin, which regulates excitatory synaptic strength, is specifically required for a postsynaptic form of synaptic homeostasis called synaptic scaling in both dissociated cultures and organotypic slices. Conversely, another form of synaptic homeostasis that involves changes in presynaptic quantal content occurs independently of beta(3) integrin. Our findings define the differential involvement of beta(3) integrin in two forms of synaptic homeostasis.
|Title:||Differential involvement of beta(3) integrin in pre- and postsynaptic forms of adaptation to chronic activity deprivation|
|Keywords:||beta(3) integrin, synaptic homeostasis, synaptic scaling, mEPSCs, hippocampal organotypic slices, HOMEOSTATIC SYNAPTIC PLASTICITY, RAT CA1 NEURONS, HIPPOCAMPAL SYNAPSES, EXCITATORY SYNAPSES, INHIBITORY SYNAPSES, VISUAL DEPRIVATION, QUANTAL AMPLITUDE, INACTIVITY, RELEASE, EXPERIENCE|
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