UCL Discovery
UCL home » Library Services » Electronic resources » UCL Discovery

RNA Interference-Mediated Inhibition of ESCRT in Mammalian Cells

Bowers, K; (2019) RNA Interference-Mediated Inhibition of ESCRT in Mammalian Cells. Methods in Molecular Biology , 1998 pp. 305-318. 10.1007/978-1-4939-9492-2_22. Green open access

[thumbnail of Bowers.pdf]
Preview
 Text
Bowers.pdf - Accepted Version
Download (1MB) | Preview

Abstract

Specific depletion of proteins from cultured cells using RNA interference (RNAi) has been a useful technique in assessing protein function for many years. RNAi allows the degradation of specific, targeted mRNA, allowing the effects of protein depletion on cellular processes to be examined. Here, I present a protocol for the depletion of proteins from cultured HeLa cells, and list specific reagents and considerations for targeting the endosomal sorting complexes required for transport (ESCRT).

Type: Article
Title: RNA Interference-Mediated Inhibition of ESCRT in Mammalian Cells
Location: United States
Open access status: An open access version is available from UCL Discovery
DOI: 10.1007/978-1-4939-9492-2_22
Publisher version: https://doi.org/10.1007/978-1-4939-9492-2_22
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: ESCRT, Endosomes, Protein depletion, RNAi, siRNA
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Structural and Molecular Biology
URI: https://discovery.ucl.ac.uk/id/eprint/10079177
Downloads since deposit
156Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item