Boyd, A;
Wilson, A;
Chee, M;
(2019)
Isolation and Characterisation of Human Adipose-Derived Stem Cells.
In: Boyd, A, (ed.)
Immunological Tolerance.
(pp. 3-13).
Humana Press: New York, NY, USA.
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Abstract
Recently, adipose-derived stem cells (ASCs), obtained from fresh human lipoaspirate, have shown promise as immunomodulatory agents having demonstrated immunosuppressive functionality both in vitro and in vivo. A number of researchers have described the isolation of ASCs through the enzymatic digestion of fat samples, followed by a number of purification steps, involving centrifugation and filtration. Here, we utilize a standard isolation technique, with the added purification of putative ASCs by fluorescence activated cell sorting (FACS). ASCs are an extremely valuable resource in clinical applications, including reconstruction, regeneration, and investigations into immune activity. This method could be used to isolate and purify ASCs for such downstream applications.
| Type: | Book chapter |
|---|---|
| Title: | Isolation and Characterisation of Human Adipose-Derived Stem Cells |
| ISBN-13: | 978-1-4939-8936-2 |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1007/978-1-4939-8938-6_1 |
| Publisher version: | https://link.springer.com/protocol/10.1007%2F978-1... |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. |
| Keywords: | Adipose-derived stem cells, Adipose stem cells, ASCs, Isolation, Characterization, Immunosuppression, Immunomodulation, Stromal vascular fraction, Collagenase, Lipotransfer, Lipoaspirate |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Surgery and Interventional Sci UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Surgery and Interventional Sci > Department of Surgical Biotechnology |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10077186 |
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