Ovcinnikovs, V;
Ross, EM;
Petersone, L;
Edner, NM;
Heuts, F;
Ntavli, E;
Kogimtzis, A;
... Walker, LSK; + view all
(2019)
CTLA-4-mediated transendocytosis of costimulatory molecules primarily targets migratory dendritic cells.
Science Immunology
, 4
(35)
, Article eaaw0902. 10.1126/sciimmunol.aaw0902.
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Walker_CTLA-4-mediated transendocytosis of costimulatory molecules primarily targets migratory dendritic cells_AAM.pdf - Accepted Version Download (28MB) | Preview |
Abstract
CTLA-4 is a critical negative regulator of the immune system and a major target for immunotherapy. However, precisely how it functions in vivo to maintain immune homeostasis is not clear. As a highly endocytic molecule, CTLA-4 can capture costimulatory ligands from opposing cells by a process of transendocytosis (TE). By restricting costimulatory ligand expression in this manner, CTLA-4 controls the CD28-dependent activation of T cells. Regulatory T cells (T_{regs} ) constitutively express CTLA-4 at high levels and, in its absence, show defects in TE and suppressive function. Activated conventional T cells (T_{conv}) are also capable of CTLA-4–dependent TE; however, the relative use of this mechanism by T_{regs} and T_{conv} in vivo remains unclear. Here, we set out to characterize both the perpetrators and cellular targets of CTLA-4 TE in vivo. We found that T_{regs} showed constitutive cell surface recruitment of CTLA-4 ex vivo and performed TE rapidly after TCR stimulation. T_{regs} outperformed activated T_{conv} at TE in vivo, and expression of ICOS marked T_{regs} with this capability. Using TCR transgenic T_{regs} that recognize a protein expressed in the pancreas, we showed that the presentation of tissue-derived self-antigen could trigger T_{regs} to capture costimulatory ligands in vivo. Last, we identified migratory dendritic cells (DCs) as the major target for T_{reg}-based CTLA-4–dependent regulation in the steady state. These data support a model in which CTLA-4 expressed on T_{regs} dynamically regulates the phenotype of DCs trafficking to lymph nodes from peripheral tissues in an antigen-dependent manner.
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