Atherton, J;
Stouffer, M;
Francis, F;
Moores, CA;
(2018)
Microtubule architecture in vitro and in cells revealed by cryo-electron tomography.
Acta Crystallographica Section D - Structural Biology
, D74
pp. 572-584.
10.1107/S2059798318001948.
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Abstract
The microtubule cytoskeleton is involved in many vital cellular processes. Microtubules act as tracks for molecular motors, and their polymerization and depolymerization can be harnessed to generate force. The structures of microtubules provide key information about the mechanisms by which their cellular roles are accomplished and the physiological context in which these roles are performed. Cryo-electron microscopy allows the visualization of in vitro-polymerized microtubules and has provided important insights into their overall morphology and the influence of a range of factors on their structure and dynamics. Cryo-electron tomography can be used to determine the unique three-dimensional structure of individual microtubules and their ends. Here, a previous cryo-electron tomography study of in vitro-polymerized GMPCPP-stabilized microtubules is revisited, the findings are compared with new tomograms of dynamic in vitro and cellular microtubules, and the information that can be extracted from such data is highlighted. The analysis shows the surprising structural heterogeneity of in vitro-polymerized microtubules. Lattice defects can be observed both in vitro and in cells. The shared ultrastructural properties in these different populations emphasize the relevance of three-dimensional structures of in vitro microtubules for understanding microtubule cellular functions.
| Type: | Article |
|---|---|
| Title: | Microtubule architecture in vitro and in cells revealed by cryo-electron tomography |
| Location: | Diamond Light Source, Harwell, ENGLAND |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1107/S2059798318001948 |
| Publisher version: | https://doi.org/10.1107/S2059798318001948 |
| Language: | English |
| Additional information: | This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
| Keywords: | Science & Technology, Life Sciences & Biomedicine, Physical Sciences, Biochemical Research Methods, Biochemistry & Molecular Biology, Biophysics, Crystallography, microtubules, cryo-electron tomography, three-dimensional reconstruction, lattice defects, neurons, SLOWLY HYDROLYZABLE ANALOG, ALPHA-BETA-TUBULIN, GTP HYDROLYSIS, ELECTRON-MICROSCOPY, LATTICE-DEFECTS, SURFACE LATTICE, STRUCTURAL TRANSITIONS, PROTOFILAMENT NUMBERS, DYNAMIC INSTABILITY, MECHANICAL-STRESS |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Structural and Molecular Biology |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10073370 |
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