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Structures of biomolecular complexes by combination of NMR and cryoEM methods

Cuniasse, P; Tavares, P; Orlova, EV; Zinn-Justin, S; (2017) Structures of biomolecular complexes by combination of NMR and cryoEM methods. Current Opinion in Structural Biology , 43 pp. 104-113. 10.1016/j.sbi.2016.12.008. Green open access

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Abstract

CryoEM is presently providing structures of biocomplexes considered intractable to analysis by other structural techniques. NMR is playing an important role in delivering structural information on dynamics events and conformational heterogeneity. Impressive results were obtained by combining cryoEM and either liquid- or solid-state NMR, revealing the structures of cellular machines, filaments and amyloid fibrils. NMR solution structures of proteins and nucleic acids were fitted, together with crystallographic structures, into cryoEM maps of large complexes, to decipher their assembly mechanisms and describe their functional dynamics. Modelling based on solid-state NMR and cryoEM data provided 3D structure of filaments and fibrils. These NMR approaches validated, but also corrected, atomic models built de novo in cryoEM maps, and provided new structural data on flexible or structurally heterogeneous systems. Combination of cryoEM and NMR became an established hybrid approach in structural biology that significantly contributes to our understanding of functional mechanisms in supramolecular assemblies.

Type: Article
Title: Structures of biomolecular complexes by combination of NMR and cryoEM methods
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.sbi.2016.12.008
Publisher version: https://doi.org/10.1016/j.sbi.2016.12.008
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: Science & Technology, Life Sciences & Biomedicine, Biochemistry & Molecular Biology, Cell Biology, SOLID-STATE NMR, 8 ANGSTROM RESOLUTION, III SECRETION NEEDLE, CRYOELECTRON MICROSCOPY, ATOMIC-STRUCTURE, MECHANISM, DYNAMICS, PROTEIN, VIRUS, RIBOSOME
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Structural and Molecular Biology
URI: http://discovery.ucl.ac.uk/id/eprint/10073362
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