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Opto‐epigenetic modulation of DNA methylation with a photo‐responsive small‐molecule approach

Nguyen, HP; Stewart, S; Kukwikila, MN; Jones, SF; Offenbartl-Stiegert, D; Mao, S; Balasubramanian, S; ... Howorka, S; + view all (2019) Opto‐epigenetic modulation of DNA methylation with a photo‐responsive small‐molecule approach. Angewandte Chemie International Edition 10.1002/anie.201901139. (In press).

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Abstract

Controlling the functional dynamics of DNA within living cells is essential in biomedical research. Epigenetic modifications such as DNA methylation play a key role in this process. Controlled DNA methylation editing can be attained via genetic means. Yet there are few chemical tools available for the spatial and temporal modulation of this modification. Here we present a small‐molecule approach to modulate DNA methylation with light. The strategy uses a photo‐tuneable version of a clinically used drug (5‐aza‐2‐deoxycytidine) to alter the catalytic activity of DNA methyltransferases, the enzymes that methylate DNA. After uptake by cells, the photo‐regulated molecule can be light‐controlled to reduce genome‐wide DNA methylation levels in proliferating cells. The chemical tool complements genetic, biochemical and pharmacological approaches to study the role of DNA methylation in biology and medicine.

Type: Article
Title: Opto‐epigenetic modulation of DNA methylation with a photo‐responsive small‐molecule approach
Location: Germany
DOI: 10.1002/anie.201901139
Publisher version: https://doi.org/10.1002/anie.201901139
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: epigenetics, methylation,decitabine, 5-aza-2-deoxycytidine, photo cage
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute > Research Department of Cancer Bio
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > Dept of Chemistry
URI: http://discovery.ucl.ac.uk/id/eprint/10068682
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