Krokowski, S;
Lobato-Márquez, D;
Chastanet, A;
Pereira, PM;
Angelis, D;
Galea, D;
Larrouy-Maumus, G;
... Mostowy, S; + view all
(2018)
Septins Recognize and Entrap Dividing Bacterial Cells for Delivery to Lysosomes.
Cell Host and Microbe
, 24
(6)
866-874.e4.
10.1016/j.chom.2018.11.005.
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Abstract
The cytoskeleton occupies a central role in cellular immunity by promoting bacterial sensing and antibacterial functions. Septins are cytoskeletal proteins implicated in various cellular processes, including cell division. Septins also assemble into cage-like structures that entrap cytosolic Shigella, yet how septins recognize bacteria is poorly understood. Here, we discover that septins are recruited to regions of micron-scale membrane curvature upon invasion and division by a variety of bacterial species. Cardiolipin, a curvature-specific phospholipid, promotes septin recruitment to highly curved membranes of Shigella, and bacterial mutants lacking cardiolipin exhibit less septin cage entrapment. Chemically inhibiting cell separation to prolong membrane curvature or reducing Shigella cell growth respectively increases and decreases septin cage formation. Once formed, septin cages inhibit Shigella cell division upon recruitment of autophagic and lysosomal machinery. Thus, recognition of dividing bacterial cells by the septin cytoskeleton is a powerful mechanism to restrict the proliferation of intracellular bacterial pathogens. Septins are cytoskeleton components widely recognized for their role in eukaryotic cell division. Krokowski et al. discover that septins recognize dividing bacterial cells for entrapment and delivery to lysosomes. These results reveal a fundamental danger signal used by the host cell to recognize intracellular bacterial pathogens for cellular immunity.
| Type: | Article |
|---|---|
| Title: | Septins Recognize and Entrap Dividing Bacterial Cells for Delivery to Lysosomes |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1016/j.chom.2018.11.005 |
| Publisher version: | https://doi.org/10.1016/j.chom.2018.11.005 |
| Language: | English |
| Additional information: | This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| Keywords: | cardiolipin, cytoskeleton, FtsZ, membrane curvature, septins, Shigella |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Lab for Molecular Cell Bio MRC-UCL |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10064121 |
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