UCL logo

UCL Discovery

UCL home » Library Services » Electronic resources » UCL Discovery

Somatic stem cell differentiation is regulated by PI3K/Tor signaling in response to local cues

Amoyel, M; Hillion, K-H; Margolis, SR; Bach, EA; (2016) Somatic stem cell differentiation is regulated by PI3K/Tor signaling in response to local cues. Development , 143 (21) pp. 3914-3925. 10.1242/dev.139782. Green open access

[img]
Preview
Text
DEVELOP-2016-139782v2-Bach.pdf - ["content_typename_Accepted version" not defined]

Download (9MB) | Preview

Abstract

Stem cells reside in niches that provide signals to maintain self-renewal, and differentiation is viewed as a passive process that depends on loss of access to these signals. Here, we demonstrate that the differentiation of somatic cyst stem cells (CySCs) in the Drosophila testis is actively promoted by PI3K/Tor signaling, as CySCs lacking PI3K/Tor activity cannot differentiate properly. We find that an insulin peptide produced by somatic cells immediately outside of the stem cell niche acts locally to promote somatic differentiation through Insulin-like receptor (InR) activation. These results indicate that there is a local ‘differentiation' niche that upregulates PI3K/Tor signaling in the early daughters of CySCs. Finally, we demonstrate that CySCs secrete the Dilp-binding protein ImpL2, the Drosophila homolog of IGFBP7, into the stem cell niche, which blocks InR activation in CySCs. Thus, we show that somatic cell differentiation is controlled by PI3K/Tor signaling downstream of InR and that the local production of positive and negative InR signals regulates the differentiation niche. These results support a model in which leaving the stem cell niche and initiating differentiation are actively induced by signaling.

Type: Article
Title: Somatic stem cell differentiation is regulated by PI3K/Tor signaling in response to local cues
Open access status: An open access version is available from UCL Discovery
DOI: 10.1242/dev.139782
Publisher version: https://doi.org/10.1242/dev.139782
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: Science & Technology, Life Sciences & Biomedicine, Developmental Biology, PI3K, Tor, Insulin, Stem cell differentiation, Drosophila, Testis, DROSOPHILA INSULIN-RECEPTOR, SELF-RENEWAL, GROWTH-CONTROL, MAMMALIAN TARGET, TUMOR-SUPPRESSOR, SUPPORT CELLS, LIFE-SPAN, GERMLINE, NICHE, RAPAMYCIN
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Cell and Developmental Biology
URI: http://discovery.ucl.ac.uk/id/eprint/10059399
Downloads since deposit
53Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item