Economides, G;
Falk, S;
Mercer, A;
(2018)
Biocytin Recovery and 3D Reconstructions of Filled Hippocampal CA2 Interneurons.
Journal of Visualized Experiments
, 141
, Article e58592. 10.3791/58592.
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Abstract
How cortical network activity processes information is of importance to a large number of basic 26 and clinical scientific questions. The protocol described here identifies the basic building blocks 27 of this circuitry. The in-depth studies of cortical regions will ultimately provide other scientists 28 with the circuit components needed for an understanding of how the brain acquires, processes 29 and stores information and what goes wrong in disease, while the electrophysiological and morphological data are widely used by computational neuroscientists in the construction of model networks that explore information processing. The protocol outlined here describes how biocytin-filled cells recorded in the CA2 region of the hippocampus are recovered and then reconstructed in 3D. Additionally, the protocol describes the demonstration of calcium binding protein or peptide content in recorded interneurons.
| Type: | Article |
|---|---|
| Title: | Biocytin Recovery and 3D Reconstructions of Filled Hippocampal CA2 Interneurons |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.3791/58592 |
| Publisher version: | http://dx.doi.org/10.3791/58592 |
| Language: | English |
| Additional information: | This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| Keywords: | Immunohistochemistry, immunofluorescence protocol, HRP protocol, neuronal reconstructions, Neurolucida, Camera Lucida, neuronal anatomy, dendrites, axon |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy > Pharmacology |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10056410 |
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