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Continuous enzymatic hydrolysis of sugar beet pectin and l-arabinose recovery within an integrated biorefinery

Cárdenas-Fernández, M; Hamley-Bennett, C; Leak, DJ; Lye, GJ; (2018) Continuous enzymatic hydrolysis of sugar beet pectin and l-arabinose recovery within an integrated biorefinery. Bioresour Technol , 269 pp. 195-202. 10.1016/j.biortech.2018.08.069. Green open access

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Abstract

Sugar beet pulp (SBP) fractionated by steam explosion, released sugar beet pectin (SB-pectin) which was selectively hydrolysed using a novel α-l-arabinofuranosidase (AF), yielding monomeric l-arabinose (Ara) and a galacturonic acid rich backbone (GABB). AF was immobilised on an epoxy-functionalised resin with 70% overall immobilisation yield. Pretreatment of SB-pectin, to remove coloured compounds, improved the stability of the immobilised AF, allowing its reutilisation for up to 10 reaction cycles in a stirred tank reactor. Continuous hydrolysis of SB-pectin was subsequently performed using a packed bed reactor (PBR) with immobilised AF. Reactor performance was evaluated using a Design of Experiment approach. Pretreated SB-pectin hydrolysis was run for 7 consecutive days maintaining 73% of PBR performance. Continuous separation of Ara from GABB was achieved by tangential flow ultrafiltration with 92% Ara recovery. These results demonstrate the feasibility of establishing a continuous bioprocess to obtain Ara from the inexpensive SBP biomass.

Type: Article
Title: Continuous enzymatic hydrolysis of sugar beet pectin and l-arabinose recovery within an integrated biorefinery
Location: England
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.biortech.2018.08.069
Publisher version: http://doi.org/10.1016/j.biortech.2018.08.069
Language: English
Additional information: Copyright © 2018 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY license (http://creativecommons.org/licenses/BY/4.0/).
Keywords: Arabinofuranosidase, Biomass pretreatment, Continuous bioprocessing, Sugar beet pectin, l-Arabinose
URI: http://discovery.ucl.ac.uk/id/eprint/10056090
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