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3D functional ultrasound imaging of the cerebral visual system in rodents

Gesnik, M; Blaize, K; Deffieux, T; Gennisson, J-L; Sahel, J-A; Fink, M; Picaud, S; (2017) 3D functional ultrasound imaging of the cerebral visual system in rodents. NeuroImage , 149 pp. 267-274. 10.1016/j.neuroimage.2017.01.071. Green open access

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Abstract

3D functional imaging of the whole brain activity during visual task is a challenging task in rodents due to the complex tri-dimensional shape of involved brain regions and the fine spatial and temporal resolutions required to reveal the visual tract. By coupling functional ultrasound (fUS) imaging with a translational motorized stage and an episodic visual stimulation device, we managed to accurately map and to recover the activity of the visual cortices, the Superior Colliculus (SC) and the Lateral Geniculate Nuclei (LGN) in 3D. Cerebral Blood Volume (CBV) responses during visual stimuli were found to be highly correlated with the visual stimulus time profile in visual cortices (r=0.6), SC (r=0.7) and LGN (r=0.7). These responses were found dependent on flickering frequency and contrast, and optimal stimulus parameters for largest CBV increases were obtained. In particular, increasing the flickering frequency higher than 7Hz revealed a decrease of visual cortices response while the SC response was preserved. Finally, cross-correlation between CBV signals exhibited significant delays (d=0.35s +/-0.1s) between blood volume response in SC and visual cortices in response to our visual stimulus. These results emphasize the interest of fUS imaging as a whole brain neuroimaging modality for brain vision studies in rodent models.

Type: Article
Title: 3D functional ultrasound imaging of the cerebral visual system in rodents
Location: United States
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.neuroimage.2017.01.071
Publisher version: https://doi.org/10.1016/j.neuroimage.2017.01.071
Language: English
Additional information: © 2017 The Author(s). Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/BY-NC-ND/4.0/).
Keywords: Animals, Brain Mapping, Imaging, Three-Dimensional, Male, Rats, Rats, Long-Evans, Ultrasonography, Visual Cortex, Visual Pathways
URI: http://discovery.ucl.ac.uk/id/eprint/10054213
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