UCL logo

UCL Discovery

UCL home » Library Services » Electronic resources » UCL Discovery

Verification of NADH content measurements by portable optical diagnostic system in living brain tissue

Zherebtsov, E; Angelova, P; Sokolovski, S; Abramov, A; Rafailov, E; (2018) Verification of NADH content measurements by portable optical diagnostic system in living brain tissue. In: (Proceedings) SPIE Photonics Europe, 2018. SPIE: Strasbourg, France. Green open access

[img]
Preview
Text
106854E.pdf - ["content_typename_Published version" not defined]

Download (539kB) | Preview

Abstract

The overall aim of this study was verification of the possibility to register the change of NADH fluorescence in live tissue by a portable diagnostical laser system with fibre optical probe output and excitation by compact semiconductor UV light source. The measurements were conducted in fresh brain tissue slices of Wistar rat pups. The fluorescence measurements were conducted simultaneously at intervals of 5 s by the microscopic system with excitation at 360 nm and registering of the emitted fluorescence light at 455 nm and by the tested diagnostical system equipped with the fibre optical probe with excitation at 365 nm and registration of the fluorescence spectrum by the inbuilt spectroscopic subsystem. To modulate the mitochondrial function in the living cells, in the chamber sequentially were added 1 μM FCCP and 1 mM NaCN. The comparisons between the curves registered by the methods allowed us to find well agreement between the microscopic measurements and measurements by the fibre optical probe. The obtained results prove that the tested diagnostic system is capable of sensing the changes in brain metabolic activity associated with the NADH content alterations within the physiological range.

Type: Proceedings paper
Title: Verification of NADH content measurements by portable optical diagnostic system in living brain tissue
Event: SPIE Photonics Europe, 2018
ISBN-13: 9781510618961
Open access status: An open access version is available from UCL Discovery
DOI: 10.1117/12.2307552
Publisher version: https://doi.org/10.1117/12.2307552
Language: English
Additional information: This version is the version of record. For information on re-use, please refer to the publisher’s terms and conditions.
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology > Clinical and Movement Neurosciences
URI: http://discovery.ucl.ac.uk/id/eprint/10053337
Downloads since deposit
52Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item