UCL Discovery
UCL home » Library Services » Electronic resources » UCL Discovery

A Statistically Representative Atlas for Mapping Neuronal Circuits in the Drosophila Adult Brain

Arganda-Carreras, I; Manoliu, T; Mazuras, N; Schulze, F; Iglesias, JE; Buhler, K; Jenett, A; ... Andrey, P; + view all (2018) A Statistically Representative Atlas for Mapping Neuronal Circuits in the Drosophila Adult Brain. Frontiers in Neuroinformatics , 12 , Article 13. 10.3389/fninf.2018.00013. Green open access

[thumbnail of fninf-12-00013.pdf]
Preview
Text
fninf-12-00013.pdf - Published Version

Download (6MB) | Preview

Abstract

Imaging the expression patterns of reporter constructs is a powerful tool to dissect the neuronal circuits of perception and behavior in the adult brain of Drosophila, one of the major models for studying brain functions. To date, several Drosophila brain templates and digital atlases have been built to automatically analyze and compare collections of expression pattern images. However, there has been no systematic comparison of performances between alternative atlasing strategies and registration algorithms. Here, we objectively evaluated the performance of different strategies for building adult Drosophila brain templates and atlases. In addition, we used state-of-the-art registration algorithms to generate a new group-wise inter-sex atlas. Our results highlight the benefit of statistical atlases over individual ones and show that the newly proposed inter-sex atlas outperformed existing solutions for automated registration and annotation of expression patterns. Over 3,000 images from the Janelia Farm FlyLight collection were registered using the proposed strategy. These registered expression patterns can be searched and compared with a new version of the BrainBaseWeb system and BrainGazer software. We illustrate the validity of our methodology and brain atlas with registration-based predictions of expression patterns in a subset of clock neurons. The described registration framework should benefit to brain studies in Drosophila and other insect species.

Type: Article
Title: A Statistically Representative Atlas for Mapping Neuronal Circuits in the Drosophila Adult Brain
Open access status: An open access version is available from UCL Discovery
DOI: 10.3389/fninf.2018.00013
Publisher version: http://doi.org/10.3389/fninf.2018.00013
Language: English
Additional information: Copyright © 2018 Arganda-Carreras, Manoliu, Mazuras, Schulze, Iglesias, Bühler, Jenett, Rouyer and Andrey. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Keywords: Drosophila adult brain, anatomical atlas, confocal microscopy, brain mapping, average brain template, diffeomorphic image registration, atlas-based image segmentation
UCL classification: UCL
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Med Phys and Biomedical Eng
URI: https://discovery.ucl.ac.uk/id/eprint/10053026
Downloads since deposit
69Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item