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Iterative peptide synthesis in membrane cascades: Untangling operational decisions

Chen, W; Sharifzadeh, M; Shah, N; Livingston, AG; (2018) Iterative peptide synthesis in membrane cascades: Untangling operational decisions. Computers and Chemical Engineering , 115 pp. 275-285. 10.1016/j.compchemeng.2018.04.024. Green open access

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Abstract

Membrane enhanced peptide synthesis (MEPS) combines liquid-phase synthesis with membrane filtration, avoiding time-consuming separation steps such as precipitation and drying. Although performing MEPS in a multi-stage cascade is advantageous over a single-stage configuration in terms of overall yield, this is offset by the complex combination of operational variables such as the diavolume and recycle ratio in each diafiltration process. This research aims to tackle this problem using dynamic process simulation. The results suggest that the two-stage membrane cascade improves the overall yield of MEPS significantly from 72.2% to 95.3%, although more washing is required to remove impurities as the second-stage membrane retains impurities together with the anchored peptide. This clearly indicates a link between process configuration and operation. While the case study is based on the comparison of single-stage and two-stage MEPS, the results are transferable to other biopolymers such as oligonucleotides, and more complex system configurations (e.g. three-stage MEPS).

Type: Article
Title: Iterative peptide synthesis in membrane cascades: Untangling operational decisions
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.compchemeng.2018.04.024
Publisher version: https://doi.org/10.1016/j.compchemeng.2018.04.024
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: Membrane enhanced peptide synthesis, Biopolymer, Membrane cascade, Dynamic process model
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Electronic and Electrical Eng
URI: http://discovery.ucl.ac.uk/id/eprint/10051659
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