UCL logo

UCL Discovery

UCL home » Library Services » Electronic resources » UCL Discovery

Human embryonic lung epithelial tips are multipotent progenitors that can be expanded in vitro as long-term self-renewing organoids

Nikolić, MZ; Caritg, O; Jeng, Q; Johnson, J-A; Sun, D; Howell, KJ; Brady, JL; ... Rawlins, EL; + view all (2017) Human embryonic lung epithelial tips are multipotent progenitors that can be expanded in vitro as long-term self-renewing organoids. eLIFE , 6 , Article e26575. 10.7554/eLife.26575. Green open access

[img]
Preview
Text
Caritg_Human embryonic.pdf - ["content_typename_Published version" not defined]
Available under License : See the attached licence file.

Download (7MB) | Preview

Abstract

The embryonic mouse lung is a widely used substitute for human lung development. For example, attempts to differentiate human pluripotent stem cells to lung epithelium rely on passing through progenitor states that have only been described in mouse. The tip epithelium of the branching mouse lung is a multipotent progenitor pool that self-renews and produces differentiating descendants. We hypothesized that the human distal tip epithelium is an analogous progenitor population and tested this by examining morphology, gene expression and in vitro self-renewal and differentiation capacity of human tips. These experiments confirm that human and mouse tips are analogous and identify signalling pathways that are sufficient for long-term self-renewal of human tips as differentiation-competent organoids. Moreover, we identify mouse-human differences, including markers that define progenitor states and signalling requirements for long-term self-renewal. Our organoid system provides a genetically-tractable tool that will allow these human-specific features of lung development to be investigated.

Type: Article
Title: Human embryonic lung epithelial tips are multipotent progenitors that can be expanded in vitro as long-term self-renewing organoids
Location: England
Open access status: An open access version is available from UCL Discovery
DOI: 10.7554/eLife.26575
Publisher version: http://doi.org/10.7554/eLife.26575
Language: English
Additional information: Copyright © Nikolic´ et al 2017. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
Keywords: alveolar differentiation, branching, developmental biology, human, human biology, lung development, medicine, stem cell, stem cells, tip progenitor, Animals, Cell Differentiation, Cell Proliferation, Humans, Lung, Mice, Organoids, Respiratory Mucosa, Stem Cells
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine > Respiratory Medicine
URI: http://discovery.ucl.ac.uk/id/eprint/10051175
Downloads since deposit
54Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item