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Redistribution of Adhesive Forces through Src/FAK Drives Contact Inhibition of Locomotion in Neural Crest

Roycroft, A; Szabó, A; Bahm, I; Daly, L; Charras, G; Parsons, M; Mayor, R; (2018) Redistribution of Adhesive Forces through Src/FAK Drives Contact Inhibition of Locomotion in Neural Crest. Developmental Cell , 45 (5) 565-579.e3. 10.1016/j.devcel.2018.05.003. Green open access

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Abstract

Contact inhibition of locomotion is defined as the behavior of cells to cease migrating in their former direction after colliding with another cell. It has been implicated in multiple developmental processes and its absence has been linked to cancer invasion. Cellular forces are thought to govern this process; however, the exact role of traction through cell-matrix adhesions and tension through cell-cell adhesions during contact inhibition of locomotion remains unknown. Here we use neural crest cells to address this and show that cell-matrix adhesions are rapidly disassembled at the contact between two cells upon collision. This disassembly is dependent upon the formation of N-cadherin-based cell-cell adhesions and driven by Src and FAK activity. We demonstrate that the loss of cell-matrix adhesions near the contact leads to a buildup of tension across the cell-cell contact, a step that is essential to drive cell-cell separation after collision.

Type: Article
Title: Redistribution of Adhesive Forces through Src/FAK Drives Contact Inhibition of Locomotion in Neural Crest
Location: United States
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.devcel.2018.05.003
Publisher version: https://doi.org/10.1016/j.devcel.2018.05.003
Language: English
Additional information: This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
Keywords: FAK, N-cadherin, Src, collective cell migration, extracellular matrix, focal adhesion, focal contacts, intercellular tension, neural crest, traction forces
URI: http://discovery.ucl.ac.uk/id/eprint/10050156
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