UCL logo

UCL Discovery

UCL home » Library Services » Electronic resources » UCL Discovery

One-pot, two-step transaminase and transketolase synthesis of L-gluco-heptulose from L-arabinose

Bawn, M; Subrizi, F; Lye, GJ; Sheppard, TD; Hailes, HC; Ward, JM; (2018) One-pot, two-step transaminase and transketolase synthesis of L-gluco-heptulose from L-arabinose. Enzyme and Microbial Technology , 116 pp. 16-22. 10.1016/j.enzmictec.2018.05.006. Green open access

[img]
Preview
Text
Bawn et al, EMT, 2018.pdf - ["content_typename_Published version" not defined]

Download (658kB) | Preview

Abstract

The use of biocatalysis for the synthesis of high value added chemical building blocks derived from biomass is becoming an increasingly important application for future sustainable technologies. The synthesis of a higher value chemical from L-arabinose, the predominant monosaccharide obtained from sugar beet pulp, is demonstrated here via a transketolase and transaminase coupled reaction. Thermostable transketolases derived from Deinococcus geothermalis and Dei nococcus radiodurans catalysed the synthesis of L-gluco-heptulose from L-arabinose and β-hydroxypyruvate at elevated temperatures with high conversions. β-Hydroxypyruvate, a commercially expensive compound used in the transketolase reaction, was generated in situ from L-serine and α-ketoglutaric acid via a thermostable transaminase, also from Deinococcus geothermalis. The two steps were investigated and implemented in a one-pot system for the sustainable and efficient production of L-gluco-heptulose.

Type: Article
Title: One-pot, two-step transaminase and transketolase synthesis of L-gluco-heptulose from L-arabinose
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.enzmictec.2018.05.006
Publisher version: https://doi.org/10.1016/j.enzmictec.2018.05.006
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: l-Arabinose, Transaminase, Transketolase, Hydroxypyruvate, Thermostable, l-Gluco-heptulose
URI: http://discovery.ucl.ac.uk/id/eprint/10050122
Downloads since deposit
5Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item