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Quantifying Biomolecular Binding Constants Using Video Paper Analytical Devices

Miller, BS; Parolo, C; Turbé, V; Keane, CE; Gray, ER; McKendry, R; (2018) Quantifying Biomolecular Binding Constants Using Video Paper Analytical Devices. Chemistry – A European Journal , 24 (39) pp. 9783-9787. 10.1002/chem.201802394. Green open access

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Abstract

A novel ultra‐low‐cost biochemical analysis platform to quantify protein dissociation binding constants and kinetics using paper microfluidics is reported. This approach marries video imaging with one of humankind's oldest materials: paper, requiring no large, expensive laboratory equipment, complex microfluidics or external power. Temporal measurements of nanoparticle–antibody conjugates binding on paper is found to follow the Langmuir Adsorption Model. This is exploited to measure a series of antibody–antigen dissociation constants on paper, showing excellent agreement with a gold‐standard benchtop interferometer. The concept is demonstrated with a camera and low‐end smartphone, 500‐fold cheaper than the reference method, and can be multiplexed to measure ten reactions in parallel. These findings will help to widen access to quantitative analytical biochemistry, for diverse applications spanning disease diagnostics, drug discovery, and environmental analysis in resource‐limited settings.

Type: Article
Title: Quantifying Biomolecular Binding Constants Using Video Paper Analytical Devices
Location: Germany
Open access status: An open access version is available from UCL Discovery
DOI: 10.1002/chem.201802394
Publisher version: https://doi.org/10.1002/chem.201802394
Language: English
Additional information: © 2018 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/).
Keywords: adsorption, nanoparticles, paper-based, protein-protein interaction, smartphones
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > London Centre for Nanotechnology
URI: http://discovery.ucl.ac.uk/id/eprint/10049310
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