Morimoto, MM;
Tanaka, S;
Mizutani, S;
Urata, S;
Kobayashi, K;
Okabe, S;
(2018)
In Vivo Observation of Structural Changes in Neocortical Catecholaminergic Projections in Response to Drugs of Abuse.
eNeuro
, 5
(1)
, Article e0071-17. 10.1523/ENEURO.0071-17.2018.
Preview |
Text
Morimoto_In Vivo Observation of Structural Changes in Neocortical Catecholaminergic Projections in Response to Drugs of Abuse_VoR.pdf - Published Version Download (2MB) | Preview |
Abstract
Catecholaminergic (dopamine and norepinephrine) projections to the cortex play an important role in cognitive functions and dysfunctions including learning, addiction, and mental disorders. While dynamics of glutamatergic synapses have been well studied in such contexts, little is known regarding catecholaminergic projections, owing to lack of robust methods. Here we report a system to monitor catecholaminergic projections in vivo over the timeframes that such events occur. Green fluorescent protein (GFP) expression driven by tyrosine hydroxylase promoter in a transgenic mouse line enabled us to perform two-photon imaging of cortical catecholaminergic projections through a cranial window. Repetitive imaging of the same axons over 24 h revealed the highly dynamic nature of catecholaminergic boutons. Surprisingly, administration of single high dose methamphetamine (MAP) induced a transient increase in bouton volumes. This new method opens avenues for longitudinal in vivo evaluation of structural changes at single release sites of catecholamines in association with physiology and pathology of cortical functions.
Type: | Article |
---|---|
Title: | In Vivo Observation of Structural Changes in Neocortical Catecholaminergic Projections in Response to Drugs of Abuse |
Open access status: | An open access version is available from UCL Discovery |
DOI: | 10.1523/ENEURO.0071-17.2018 |
Publisher version: | https://doi.org/10.1523/ENEURO.0071-17.2018 |
Language: | English |
Additional information: | © 2018 Morimoto et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/). |
Keywords: | axon, catecholamine, in vivo imaging, methamphetamine, neocortex, transgenic mouse |
UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > Div of Psychology and Lang Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > Div of Psychology and Lang Sciences > Experimental Psychology |
URI: | https://discovery.ucl.ac.uk/id/eprint/10048796 |
Archive Staff Only
View Item |