Mittal, A;
Hobor, F;
Zhang, Y;
Martin, SR;
Gamblin, SJ;
Ramos, A;
Wilson, JR;
(2018)
The structure of the RbBP5 β-propeller domain reveals a surface with potential nucleic acid binding sites.
Nucleic Acids Research
, 46
(7)
pp. 3802-3812.
10.1093/nar/gky199.
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Abstract
The multi-protein complex WRAD, formed by WDR5, RbBP5, Ash2L and Dpy30, binds to the MLL SET domain to stabilize the catalytically active conformation required for histone H3K4 methylation. In addition, the WRAD complex contributes to the targeting of the activated complex to specific sites on chromatin. RbBP5 is central to MLL catalytic activation, by making critical contacts with the other members of the complex. Interestingly its only major structural domain, a canonical WD40 repeat -propeller, is not implicated in this function. Here, we present the structure of the RbBP5 -propeller domain revealing a distinct, feature rich surface, dominated by clusters of Arginine residues. Our nuclear magnetic resonance binding data supports the hypothesis that in addition to the role of RbBP5 in catalytic activation, its -propeller domain is a platform for the recruitment of the MLL complexes to chromatin targets through its direct interaction with nucleic acids.
| Type: | Article |
|---|---|
| Title: | The structure of the RbBP5 β-propeller domain reveals a surface with potential nucleic acid binding sites |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1093/nar/gky199 |
| Publisher version: | http://dx.doi.org/10.1093/nar/gky199 |
| Language: | English |
| Additional information: | © The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Structural and Molecular Biology |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10047400 |
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